[关键词]
[摘要]
目的 ： 研究人SCF基因转染NK细胞系的生物学特性。方法：利用LipofecTAMINE将SCF真核表达载体pcDNA3-hSCF转染NK-92细胞系,RT-PCR鉴定表达SCF的细胞克隆并以SCF依赖细胞株TF-1测活，MTT法检测增殖和杀伤活性。流式细胞术检测细胞表面分子CD3，CD16和CD56。结果：建立表达hSCF基因的NK-92-hSCF细胞株，在IL-15培养条件下，其增殖能力显著高于NK-92，并且低剂量的IL-15可以维持其较高的杀伤活性流式细胞术检测细胞表面分子CD3、CD16和CD56。结论：可以通过SCF基因修饰NK-92，改变其生物学特性，提高其增殖能力，使NK细胞系有更实用的应用价值。
[Key word]
[Abstract]
Objective:To study the characterization of NK cell line transfected with hSCF gene.Methods:pcDNA3-hSCF was transfected into NK-92 cell line with LipofectAMINE ,RT-PCR was used to identify NK-92-hSCF cell which express hSCF and the activity was assayed by TF-1 cell line. CD3, CD16 and CD56 molecules were tested by FACS. Results: We established NK-92-hSCF cell line which express hSCF steadily, and its proliferation increased compared with parental cell line when incubated with rhIL-15 or rhIL-2. Conclusion: The characterization of NK-92 could be changed by transfecting with hSCF gene, and it′s proliferation was improved, The gene transfection of NK-92 cell made it suitable for clinical applications.
[中图分类号]
R392.12
[基金项目]
国家高技术研究发展专项经费资助（编号 2002AA216151）
