[关键词]
[摘要]
目的:探索重组腺病毒介导的外源性野生型p53cDNA(Ad-wtp53)对内源性p53基因状态不同结直肠癌细胞的抑制作用是否存在差异,研究p53基因抑制肿瘤细胞增殖的机制。方法:采用MTT法选择Ad-wtp53的最佳转染滴度,分别感染内源性p53基因缺失、突变和正常的3种结直肠癌细胞株,观察比较它们的生长抑制作用。结果:Ad-wtp53在1 000 MOI时表现出最强的细胞抑制作用,p53缺失细胞株(THC-8908)的抑制效应最明显,3种细胞转染后均发生G0/G1期阻滞,并对不同p53状态细胞G2-M期具有不同的调控作用。结论:外源性野生型p53 cDNA导入可显著抑制结直肠癌细胞生长,使细胞周期停滞于G0/G1期;同时对内源性p53状态不同细胞的生长抑制强度和G2-M期调控作用不同
[Key word]
[Abstract]
Objective:To explore the inhibition effects of ectogenic wild-type p53 cDNA(Ad-wtp53) on colorectal carcinoma cell lines with different p53 gene status and search for the role of wild type p53 tumor suppressor gene in occurrenc and progress of malignant tumor.Methods:MTT process was taken to choose optimal transfection titre. Three kinds of cell lines(p53 gene deletion, mutation and nomal) were transferred by Ad-wtp53 in optimal titre. The inhibition effects of these cell lines were observed and compared. Results:The best titre is 1000 MOI and p53 gene deletion cell line (THC-8908) shew the highest sensitivity. G1-S transition period blocking effects occurred in all cell lines and G2-M phase regulation effects were not coincidence in three colorectal cell lines. Conclusions:Recombinant adenovirus-mediated wild type p53 gene observably inhibited colorectal carcinoma cell lines growth and proliferation, blocked cell cycle in G0/G1 phase and displayed obvious different actions on G2-M phase among cell lines with different p53 status.
[中图分类号]
R735.2
[基金项目]