目的: 研究全反式维甲酸（ATRA）抑制视网膜母细胞瘤（Rb）细胞生长的作用及信号转导机制。方法:应用3H-胸腺嘧啶掺入分析法观察ATRA对细胞生长的抑制作用，用流式细胞仪分析ATRA对Y79细胞周期的影响，用Western blot分析c-jun氨基末端激酶（JNK）的磷酸化。结果: ATRA可明显地抑制Y79细胞的生长，10-6 mol·L-1 ATRA处理36 h时，3 H-胸腺嘧啶掺入率下降达40%，此条件下，Y79细胞被阻滞于G0/G1期，并出现Sub-G1峰；此生长抑制过程可被JNK的阻断剂Curcumin阻断；在此过程中，JNK被激活，磷酸化。结论:ATRA可抑制Y79细胞生长，其过程是由磷酸化的JNK介导。提示，ATRA可能是一种潜在的抗视网膜母细胞瘤治疗药物。

Objective: To investigate all trans retinoid Acid (ATRA) inhibition of cell growth in human retinoblastoma Y79 cells, and its mechanism. Methods:Antiproliferation effects of ATRA on Y79 cells were determined by 3H-thymidine incorporation. Cell cycle analysis was performed by flow cytometry. JNK phosphorylation was analyzed by Western blot analysis.Results: After 36 h treatment with 10-6mol·L-1 ATRA, 3H-thymidine incorporation decreased to 40%, under the same condition, Y79 cells were arrested in G0/G1 and Sub-G1 peak appeared. Curcumin, JNK blocker, blocked the growth inhibition by ATRA. JNK was phosphorylated in 10 to 20 min. Conclusion: JNK-phosphorylating mediated ATRA inhibition of apoptosis in Y79 cells. These results suggested that ATRA might have clinical application for treatment of retinoblastoma.

R735.34