目的：探讨丝裂霉素C（MMC）对肝癌细胞中NF-κB分布和基因表达的影响。方法：应用细胞免疫组织化学检测不同浓度的MMC对SK-HEP-1细胞中NF-κB分布的影响，Western-blot检测50 μg/ml MMC作用不同时间的NF-κB蛋白水平，RT-PCR方法比较50 μg/ml MMC持续处理细胞不同时间和作用2 h停止用药后不同时间NF-κB基因表达水平的影响。结果：不同剂量的MMC能够不同程度地使细胞核中NF-κB免疫组化染色增强，50 μg/ml的MMC导致了NF-κB蛋白蛋白印迹水平升高，并在MMC处理8 h的细胞中升至最高，RT-PCR方法检测NF-κB基因mRNA水平在MMC持续处理的细胞中也上升，在第2小時水平最高，随后下降，但是在MMC处理2 h停止用药后NF-κB基因mRNA水平没有下降反而继续上升，并持续到第12小時。结论：MMC能够促进NF-κB自细胞浆进入细胞核，50 μg/ml的MMC导致了NF-κB自身表达升高并在撤除用药后仍旧存在，提示NF-κB同细胞抗MMC活性密切相关。

Objective：To detect the effect of Mitomycin-C (MMC) on expression level and cellular distribution of NF-κB after MMC stimulation to further understand the mechanism of the NF-κB signal transduction in the response of SK-HEP-1 cell to the chemical drug. Methods：The cellular distribution and protein level of NF-κB was detected by Immunohistochemistry and Western blot method in SK-HEP-1 cell treated with different concentration of MMC for different duration. The mRNA expression of NF-κB gene was determined by RT-PCR in SK-HEP-1 cell treated with 50 μg/ml concentration MMC for different time after continuing treatment and 2 h treatment.Results：MMC stimulated the NF-κB influx into the nucleus from plasma and elevated the protein level of NF-κB and reached the peak at 8th hour. On effect of continual treatment of 50 μg/ml concentration MMC the expression level of NF-κB gene was elevated until the peak at 2nd h and then descended. However after treatment of MMC for 2 h and then withdrawal, the expression of NF-κB was elevated until 12th h. Conclusions：MMC could drive the NF-κB to come into the nucleus and elevate the expression of the gene. It was suggested that the NF-κB signal transduction pathway had relation to the response of SK-HEP-1 cell to MMC treatment.

国家“863”项目（2001AA221021）资助