目的：研究肺癌清扫淋巴结的淋巴细胞，经CD3单克隆抗体激活后的杀伤细胞（CD3AK细胞）在体外的增殖和表型变化。方法：取肺癌清扫淋巴结，用机械方法分离成单细胞悬液，加CD3单克隆抗体和小剂量IL 2共同培养，每隔1天用流式细胞术进行细胞表型分析及用台盼蓝活细胞计数法观察细胞增殖情况。结果：表型分析显示CD3，CD8，CD56，CD25增加。培养第8天淋巴细胞增殖达高峰，数量是培养前的2.33倍。结论：CD3单克隆抗体能明显激活各种T细胞增殖，使CD3AK细胞维持杀伤功能。

Objective:To study the propagation and phenotypes changes of killer cell (CD3AK cell activated by CD3 mAb in vitro.Methods: Lymph nodes taken from lung cancer patient is dissociated into single cell suspension by mechanical method and cultured in culture medium added CD3 mAb and a little dose IL-2. We analyze cell immunophenotype by flow cytometry and proliferation by trypan blue exclusion test per 2 days. Results: Immunophenotypic analysis showed that CD3AK expressing CD3, CD8, CD56, CD25 increased, and reached a peak value which is 2.33 times than before culturing in the 8 th day. Conclusion: CD3 mAb added to the culture medium can obviously activate CD3AK cell and stimulate proliferation and keep its killer activity.

本课题受浙江省医药卫生科研基金资助（基金编号96001）