目的：探讨肺癌患者外周血来源的树突状细胞（dendritic cells, DC）生物学特性。方法：从肺癌患者外周血分离获得单个核细胞，用细胞因子GM-CSF (100 μg/L)，IL-4 (50 μg/L)体外培养诱导。凋亡肿瘤细胞负载24 h后加入TNF-α（10 μg/L）或CD40激发型单抗于培养DC中，继续诱导3～4 d。分别测定DC的表型、DC摄取抗原能力；混合淋巴细胞反应（MLR）对T细胞增殖能力的测定；细胞计数和3H-TdR掺入观察DC对T细胞的激发和扩增效应，并与健康志愿者外周血来源的DC进行比较。结果： 患者外周血单个核细胞和正常人外周血单个核细胞来源的DC均高表达CD1α，CD83，CD80，CD86和HLA-DR等DC的相关抗原和共刺激分子；患者的未成熟DC能有效摄取FITC-Dextran，经TNF-α或CD40激发型单抗激发诱导后，成为成熟和有功能的DC,几乎完全失去对抗原的摄取能力，与健康人外周血来源DC组相比无明显差别(P>0.05)；患者单个核细胞来源的DC在体外具有激发自体和同种异体外周血T细胞增殖的能力。结论：肺癌患者的外周血来源单个核细胞可以诱导成为具有功能的成熟DC。

Objective：To study the biological characteristics of dendritic cells (DC) derived from peripheral blood of patients with lung cancer.Methods：Peripheral blood mononuclear cells (PBMC) were isolated from 11 lung cancer patients and healthy donors respectively, cultured with GM-CSF (100 μg/L) and IL-4 (50 μg/L). After pulsed with apoptotic lung cancer cells for 24 h, TNF-α（10 μg/L）added or agonist CD40mAb (5 mg/L) for a further induction of 3～4 days. Phenotypes of DC were detected by FCM. The capacities for DC to uptake antigens were monitored at day 7 and day 11 detected by FITC-dextran uptake test.3H-thymidine incorporation test was used to measure the proliferative capability of T cells. Results： Both DC derived from PBMC of lung cancer patients and from PBMC of healthy donors showed high expressions of CD1α, CD80, CD83, CD86 and HLA-DR, which were known as associated markers of mature DC. The 7th day DC derived from lung cancer patients PBMC showed a strong capacity to uptake antigens. DC were induced by TNF-α or CD40mAb, then became mature. DC entirely lost their capacities of uptake antigens, while DC could stimulate autologous and allogenic T cells' proliferation. When matured DC co-incubated with autologous T cells for 48 h, activated T cells could be observed and the number of activated T cells was significantly increased (P<0.05).Conclusion：The functional and mature DC could be induced from peripheral blood of lung cancer patients in vitro.

江苏省社会发展基金（BS2000038）和江苏省医学重点人才基金（RC2002032）