目的：研究ets-1反义寡核苷酸对胃癌的治疗作用及其机制。方法：应用ets-1反义、正义寡核苷酸转染SGC-7901细胞，并设PBS对照组。转染后应用逆转录聚合酶链式反应检测ets-1mRNA的变化，克隆形成试验检测细胞增殖活性的变化，Transwell小室检测癌细胞体外侵袭力的变化，并应用裸鼠胃癌皮下转移模型观察对体内侵袭力的影响。结果：转染ets-1反义寡核苷酸后，ets-1mRNA表达降低，形成克隆数目较正义组、对照组明显减少（24.2±4.8 vs 47.6±8.1 vs 44.3±7.6，P<0.01），穿过小室滤膜细胞数目明显减少（97.1±11.1 vs 198.7±18.3，205.8±22.2，P<0.01），裸鼠胃癌生长明显受到抑制。结论： ets1反义寡核苷酸对胃癌有治疗作用，其机制可能与降低胃癌细胞体内外侵袭力有关。

Objective: To investigate the therapeutic effects of ets-1 antisense oligoxydeonucleotide(AsODN) on gastric carcinoma. Methods: Cultured SGC-7901 cells were devided into control group, sense oligoxydeonucleotide(sODN) group and AsODN group. After transfection for 24 h, expression of ets-1mRNA was detected by RT-PCR, growth inhibition was detected by colone formation assay, in vitro invasive ability assay was carried out in Transwell chamber，the animal model of xenotransplanted gastric carcinoma in nude mice was established to detect invasive ability in vivo. Results: ets-1 AsODN could under-regulate the expression of ets-1 mRNA, number of colone formation of AsODN group was significantly lower than the other two groups(24.2±4.8 vs 47.6±8.1 vs 44.3±7.6，P<0.01）, so was the number of cells which crossed to the lower surface of the matrigel-coated filters (97.1±11.1 vs 198.7±18.3, 205.8±22.2， P<001）. ets-1 AsODN could also inhibit the growth of xenotransplanted gastric carcinoma in nude mice significantly. Conclusion: ets-1 AsODN had therapeutic effects on gastric cancinoma, and the inhibition of the invasive ability of gastric cancer cells might be one of its mechanisms.