目的：分析实体瘤患者外周血中的循环血管内皮细胞（circulating endothelial cells, CECs）及血管内皮前体细胞(circulating endothelial precursors，CEPs)，并比较与正常人的差异。方法：流式细胞法检测57肿瘤患者及15例正常人外周血中的CECs和CEPs，ELISA法检测血清中血管生成相关因子VEGF和bFGF的表达水平。结果：肿瘤患者外周血CECs及CEPs分别为(0.378±0.047)%和(0.059±0.013)%，血清VEGF，bFGF分别为(295.58±59.56) ng/L和(28.73±7.40) ng/L，均高于正常对照组（P<0.05）。血管内皮及其前体细胞的数量与血清VEGF，bFGF水平相关。结论：实体瘤患者外周血中循环血管内皮及其前体细胞、血管内皮生长因子均高于正常人；VEGF和bFGF可能参与了血管内皮干/祖细胞的动员过程。

Objective:To analyze the circulating endothelial cells (CECs) and circulating endothelial precursors (CEPs) in peripheral blood of tumor patients. Methods:CECs and CEPs were enumerated in 57 tumor patients and 15 healthy controls by 3-color flow cytometry. The serum level of angiogenesis related cytokines（VEGF, bFGF）was determined by ELISA method.Results：In tumor patients, mean value of CECs and CEPs was (0.378±0.047)% and (0059±0.013)% respectively. The serum level of VEGF and bFGF were (295.58±59.56) ng/L and (28.73±740) ng/L. The amount of CECs/CEPs positively correlated with the serum level of VEGF and bFGF. Conclusions: The CECs/CEPs and serum level of VEGF and bFGF of tumor patients was higher than those of normal controls. VEGF and bFGF may participate into the course of endothelial progenitors mobilization.

天津市自然科学基金（98311351）