目的：研究黄芪对S180肿瘤培养上清免疫抑制作用的影响。方法：用有或无中药作用的肿瘤培养上清，以MTT法检测其对小鼠脾细胞转化、IL 2产生、NK杀伤活性及CTLL-2细胞IL-2反应性的影响，以流式细胞仪检测其对小鼠脾细胞表面IL-2Rα表达的影响。结果：单纯肿瘤上清可强烈抑制所测5项指标。在单纯肿瘤上清中加入中药可明显上调上述5项指标。先用含中药培液培养肿瘤细胞，而后洗去或不洗去中药再培养肿瘤细胞所得的上清，也可明显上调所测5项指标。结论：黄芪可以直接对抗肿瘤上清的免疫抑制作用；下调S180肿瘤细胞合成和/或分泌免疫抑制物质

Objective:To investigate the effects of AMB(Astragalus Mongholicus Bge) on the immunosuppression of tumor cell line S180 supernatant.Methods:The effects of the supernatant of S180 cultured with or without AMB on the transformation, IL-2 production and NK activity of murine splenocytes and the reactivity of CTLL-2 to IL-2 were measured by MTT, and the effect on the IL-2Rα expression of murine splenocyte was detected by flow cytometry. Results: The supernatant of S180 alone could strongly inhibit the five tests. Adding AMB to the superanant of S180,the results of the five tests were up-regulated substantially.The superanants of S180 which were cultured first in AMB for 24 h or 48 h,then washing off AMB or not,and recultured for 24 h remarkably up-regulated the five tests. Conclusions:AMB could reverse the immunosuppressions of the supernatant of S180,down-regulate the synthesis and/or secretion of immunosuppressive subsance by tumor cells.