目的：研究肿瘤相关抗原编码基因CHP2对细胞增殖作用的影响。方法：采用PCR方法获得目的基因，克隆至真核表达载体pcDNA3，对产生的重组子进行酶切鉴定及DNA序列测定；测序正确的重组子用脂质体转染的方法转入293细胞系，药物G418筛选稳定转染细胞株，分别在基因水平以及蛋白水平进行鉴定；用3H-TdR掺入的方法检测CHP2对细胞增殖的影响。结果：经琼脂糖凝胶电泳及测序鉴定，所插入的片断方向及序列完全正确；Western blotting结果显示，获得的稳定转染细胞株能够高效的表达CHP2蛋白。细胞增殖实验结果显示CHP2稳定转染细胞株的3H-TdR掺入值明显低于对照组。结论：CHP2在细胞中的有效表达能够显著显著抑制细胞的增殖，CHP2可能是参与细胞生长调控的一个重要的调节因子。

Objective: To study the effect of cell proliferation transfected with tumor associated antigen encoding gene CHP2. Methods: Using PCR method to clone CHP2 encoding gene and inserting it into the eukaryotic expression vector pcDNA3 to construct the recombination pcDNA-CHP2. The fusion gene was identified by enzyme digestion and DNA sequencing. 293 cells transfected CHP2 genes were selected by G418 pressure and the cell proliferation was measured by 3-TdR uptake. Results: Identified by enzyme digestion and sequencing, CHP2 gene was correctly cloned into pcDNA3. The results of the western blot showed the transfected cell could express CHP2 protein. 3-TdR uptake value of the CHP2 transfected cell was lower than the control. Conclusions: CHP2 could inhibit cell proliferation and it maybe an important regulator involved in cell proliferation.

国家“973”基金资助项目（No. G1999053904)和北京市自然科学基金资助项目）NO. 7001002)