目的:探讨食管癌组织中AKT和PTEN的表达及其临床相关性。方法:应用Western blot和免疫组织化学方法检测42例食管癌组织及其癌旁组织中AKT,PTEN蛋白的表达情况。结果:食管癌组织中，AKT蛋白水平高于癌旁组织（P<001），PTEN蛋白水平低于癌旁组织（P<001） ，二者呈负线形相关（r=-0.583，P<0.01）。AKT蛋白表达在Ⅲb-Ⅳ期、低分化、有淋巴结转移的食管癌中的表达高于Ⅱ-Ⅲa期、高分化、无淋巴结转移的食管癌（P<001），而PTEN则完全相反（P<001）。二者的表达均与年龄、性别、肿瘤大小无关（P>005）。结论: AKT蛋白的高表达在食管癌的发生、发展起重要作用，而PTEN则抑制这一作用过程。

Objective:To investigate the expression of AKT and PTEN protein and their relationship with clinic characteristics of esophageal carcinoma.Methods: Expression of AKT and PTEN protein was examined by Western Blot and immunohistochemical method in the esophageal carcinoma and normal esophageal (control) tissue of 42 patients.Results: In tumor specimen, expression of AKT protein was increased as compared with control tissue(P<0.01), while PTEN protein was reduced compared with control tissue(P<0.01). A negative correlation was observed between AKT and PTEN(r=-0.583，P<0.01). Protein level of AKT was higher in Ⅲb-Ⅳ stage patients with low differentiatition and lymph node metastasis than those with Ⅱ-Ⅲastage, high defferentiatition and no lymph node metastasis(P<0.01). However, PTEN protein level was entirely contrary. Both of them were not related with age, sex and size of tumor(P>0.05).Conclusions: The hyperexpression of AKT may play an important role in the initiation and development of human esophageal carcinoma, while PTEN inhibit this process.