目的：观察罗勒多糖对肿瘤细胞不同途径转移行为的影响，并从细胞间通讯功能的恢复以及体内NK细胞活性的变化两方面探讨其抗肿瘤转移的可能作用机制。方法：Metrigel穿膜法体外观察罗勒多糖对肿瘤细胞移动活性的影响；建立不同途径的肿瘤转移模型，体内观察罗勒多糖对肿瘤转移行为的影响。SLDT技术检测罗勒多糖对细胞通讯功能的影响； 51Cr释放法检测罗勒多糖对NK细胞活性的影响。结果：罗勒多糖在体内外均具有显著的抗肿瘤转移作用；与对照组相比细胞间通讯功能有一定程度的恢复； NK细胞的活性明显提高。结论：罗勒多糖具有明显的抗肿瘤转移作用，可能是一种具有潜在开发价值的新的抗肿瘤转移药物，其抗肿瘤转移作用是通过多个药效靶点综合作用的结果。

Objective: To study the effect of basil polysaccharide (BP) on tumor cell metastasis and its mechanism through assays of intercellular communication and NK cytotoxicity.Methods: Metastasis of tumor cell ability was assayed by metrigel in vitro and the rate of pulmonary metastasis nodel was caluated. Using SLDT technique to test the effect of BP on recovering the function of cell Gap junction mediated intercellular communication (GJIC). NK cytotoxicity was measured by a standard 4 h 51 Cr-release assay. Results:Invasion and migration of tumor cells decreased significantly after treated with BP in vitro and in vivo.PG cells treated with BP for 8 h could recover the function of GJIC compared with the control. NK activity of mice bearing tumor treated with BP rose significantly. Conclusions: The resutls suggested that BP could inhibit tumor metastasis and might be a new candidate for antitumor metastasis agent.

R730.54 Q784

本课题由山东省卫生厅（课题编号2001CA1CDA1）以及山东省中药管理局（课题编号99-46）资助