目的：观察雷公藤内酯醇是否能抑制肿瘤细胞增殖，并探讨其是否通过诱导肿瘤细胞凋亡发挥抗肿瘤作用。方法：选择人宫颈癌细胞株HeLa为研究对象，以MTT实验检测肿瘤细胞的增殖，以AnnexinV/PI染色检测细胞的凋亡，R123染色检测线粒体膜电势的变化。Hoechst 33258荧光染色法观察细胞形态学改变，琼脂糖凝胶电泳检测染色体DNA断裂。结果：雷公藤内酯醇能明显抑制HeLa细胞的增殖；AnnexinV/PI染色结果证明雷公藤内酯醇能诱导HeLa细胞凋亡，R123染色结果说明雷公藤内酯醇诱导的细胞凋亡与线粒体膜电势的变化有关。雷公藤内酯醇处理的HeLa细胞可见凋亡特有的形态学及生物化学改变，DNA电泳呈梯状条带。结论：雷公藤内酯醇可以通过诱导肿瘤细胞凋亡而发挥抗肿瘤作用。

Objective: To investigate the proliferation inhibitory effect and the apoptosis-inducing activity of triptolide on HeLa cells. Methods: The effects of triptolide on cell proliferation were observed in vitro by methyl thiazolyl tetrazolium (MTT) method. The morphological alterations were confirmed by Hoechst 33258 staining. The apoptosis was evaluated by flow cytometry analysis and agarose gel electrophoresis. Results: Triptolide exhibited a marked antiproliferative effect on HeLa cells. Triptolide induced apoptosis of the cultured cells in a time- and dose-dependent manner, as evidenced by an increased percentage of annexin V/PI positive cells. Correspondingly, we observed a decrease of R123 uptake in Triptolide-treated cells, indicative of a reduction in mitochondrial membrane potential. The morphological changes of apoptotic cells and ladder pattern of DNA were observed in triptolide-treated cells. Conclusion: Triptolide can inhibit the proliferation of HeLa cells by induction of cells apoptosis.

R730.59

国家自然科学基金（30121002）