目的：获取抗肿瘤坏死因子相关凋亡诱导配体（TRAIL）抗体。方法：利用PCR技术和基因重组技术将TRAIL基因构建于原核表达载体pPreTMH-Tb中，经酶切、测序鉴定证实构建完全正确后，通过IPTG诱导在大肠杆菌DH-5α中获得表达，并将TRAIL蛋白免疫家兔，通过Dot blot及Western blot检测抗TRAIL抗体的产生。结果：成功构建TRAIL基因原核表达载体，并在大肠杆菌DH-5α中获得表达，TRAIL蛋白表达量占菌体蛋白质总量的11.8%，Dot blot及Western blot检测证实获得了抗TRAIL抗体。结论：成功制备抗TRAIL抗体，这为TRAIL在真核细胞水平研究奠定了实验基础。

Objective:To obtain the anti-TRAIL antibody. Methods:A prokaryotic expression vector pPre-TMHTb was constructed by PCR and recombinant DNA techniques and was confirmed by enzymatic digestion and sequence analysis. TRAIL protein induced to express by IPTG in E.coli DH-5α. After TRAIL protein immunised rabbits, anti-TRAIL antibody was tested by Dot blot and Western blot. Results:A prokaryotic expression vector pPre-TMHTb was constructed and expressd in E.coli DH-5α. TRAIL protein was detected up to 11.8% of the total bacterial protein expressed and anti-TRAIL antibody was tested by Dot blot and Western blot. Conclusions: The anti-TRAIL antibody was obtained that provides an experimental basis for the studies of TRAIL in eukaryotic level.

R282 R730.52

贵州省省长专项（No. S2001-14）资助