树突状细胞(DC)广泛分布于体内，是机体功能最强的抗原递呈细胞(APC)，在免疫应答中起着桥梁作用。最近有文献报道，白血病细胞经诱导后能分化为DC，从而启动特异性的机体抗白血病免疫应答，使白血病的治疗取得了可喜进展。将白血病细胞诱导成为具有递呈肿瘤抗原特性的DC，激发自体特异性细胞毒性T淋巴细胞的产生，对白血病细胞产生杀伤作用，表明白血病细胞来源的DC的诱导方案，其细胞生物学特性及白血病的治疗具有广泛的应用前景。

Objective: To study the selective killing effects of immunotoxin BDI-1-PEA against human-bladder cancer cell line E-J and nude mice bearing human bladder tumor. Methods; MTT was used to measure the killing ratio of BDI-1-PEA, mixtures of BDI-1 and PEA, PEA against human-bladder cancer cell line E-J at different concentrations in vitro. When the tumor size reached 0. 3 cm, BDI-1-PEA,BDI-1 and IgG were injected respectively to each group of mice though tail vein every other day for 6 times. Determined the inhibitory effects of BDI-1-PEA, BDI-1 and IgG in nude mice bearing human bladder tumor. Results; The results indicated that BDI-1 -PEA showed stronger cytotoxicity against human bladder cancer cell line E-J than PEA and mixtures of BDI-1 and PEA, and stronger cytotoxicity against nude mice bearing human bladder tumor than BDI-1 and IgG. Compared with cytotoxicity against non-target cell, the difference was significant ( P < 0.01). Conclusion: Immunotoxin BDI-1-PEA is a largely potential anticancer drug, the result is a good foundation for the further clinical study.

R737.14

本课题受江苏省自然科学基金项目（BI98100）资助