目的：探讨ehCGβ肿瘤基因疫苗诱生的效应脾细胞过继免疫在抗肿瘤中的作用。方法：通过转染建立稳定表达ehCGβ和HBV-preS2/S的细胞株Sp2/0-ehCGβ和Sp2/0-preS2/S。以TR421-hCGβ质粒实施基因免疫，免疫后检测小鼠脾细胞，特异性细胞毒活性；同期将脾细胞过继免疫给正常小鼠，以过继TR421-hCGβ质粒免疫小鼠脾细胞为实验组、过继TR421质粒免疫小鼠脾细胞为对照组，检测脾细胞杀伤效应。结果：效应脾细胞对Sp2/0-ehCGβ的杀伤率明显高于Sp2/0-preS2/S（P<0.05）。Sp2/0-ehCGβ在实验组小鼠仅2只形成实体瘤，TR421-hcGβ质粒基因免疫抗肿瘤任用有肿瘤特异性，两组有显著性差异（P<0.05），而在对照组小鼠均形成实体瘤。Sp2/0-preS2/S在所有的小鼠均形成了实体瘤，其瘤重无显著性差异。结论：ehCGβ肿瘤基因疫苗诱生的效应细胞可特异性杀伤肿瘤，过继免疫效应细胞能使正常小鼠获得明显的特异性抗肿瘤能力。

Objective：To investigate the anti-tumor effects of adoptively transferred splenocytes induced by gene immunization with ehCGβ tumor vaccine. Methods：Sp2/0 cells were transfected with the plasmids containing ehCGβ or HBV-preS2/S and the postive clones were screened by G418. BALB/c mice were immunized with plasmid TR421-hCGβ or mock DNA three times. Two weeks after immunization, spleen cells from the immunized mice were harvested, and then analyze the CTL activity against Sp2/0-ehCGβ cells. The splenocytes derived from the immunized mice were adoptively transferred to the normal mice, which were subsequently given injections i.p. of Sp2/0-ehCGβ and β Sp2/0-preS2/S respectively. Results：Splenocytes derived from the mice immunized with TR421-hCGβ exhibited a strong specfic cytotoxic activity against Sp2/0-ehCGβ cells, The average tumor weight between test and control group is statistically significant (P<0.05). Only two mice out of six had tumors in Sp2/0-ehCGβadoptively transferred group, while all mice had tumors in control group. However, all mice challenged with Sp2/0-preS2/S formed tumors, and the average tumor weight was not obviously varied between the two groups. Conclusion：ehCGβ gene vaccine could induce specific cytotoxic splenocytes against ehCGβ and adoptive transfer of the splenocytes showed the anti-tumor activity.

上海市科技攻关重点项目(03dz19229)和国家杰出青年科学基金研究计划(39925031)