目的：探讨抗肿瘤坏死因子相关凋亡配体的死亡受体DR5单克隆抗体对神经胶质瘤细胞株Ｕ343的杀伤作用及机制。方法：采用流式细胞仪从蛋白质水平定量地检测DR5在Ｕ343的表达;用RT-PCR从mRNA水平检测DR5的表达；免疫细胞化学法明确DR5在Ｕ343的分布情况。通过MTT法、琼脂糖凝胶电泳、流式细胞仪DNA倍体分析，观察抗人DR5单克隆抗体对Ｕ343细胞增殖的抑制活性及其诱导凋亡效应。结果：神经胶质瘤细胞株Ｕ343表达死亡受体DR5，DR5分布于细胞内细胞核的周围。抗人DR5单克隆抗体3 μg/ml作用4 h可明显杀伤Ｕ343细胞，其机制与诱导凋亡有关。结论：抗人DR5单克隆抗体可以诱导神经胶质瘤细胞株Ｕ343凋亡,以死亡受体为靶点的抗体制剂为肿瘤治疗提供新的途径。

Objective: To investigate the inhibitory effects and mechanism of a novel anti-human DR5(death receptor 5 of TRAIL) monoclonal antibody to glioma cell lines U343.Methods:DR5 protein was tested quantitative through FCM; DR5 mRNA was observed through RT-PCR and distribution was tested by immunocytochemistry. Inhibitory effects and inducing apoptosis of anti human DR5 monoclonal antibody to U343 were analysed by MTT, DNA Ladder,FCM. Results: The expression of death receptor 5 (DR5) was certificated in U343,DR5 appeared to be located in intracellular perinuclear compartment. Inhibitory effects of anti-human DR5 monoclonal antibody on U343 were achieved by 3 μg/ml at 4 hours and the mechanism was associated with apoptosis. Conclusion: Apoptosis of glioma cell lines U343 can be induced by anti-human DR5 monoclonal antibody, and targeted on DR will provide new way to treating cancer.

教育部《跨世纪优秀人才培养计划》资助项目