目的: 验证环氧化酶-2（COX-2）启动子能调控下游基因特异性在COX-2阳性的卵巢癌细胞系高表达；并以CMV启动子为对照，分析COX-2启动子的转录效率。方法: 构建重组质粒COX-2-BAX和CMV-Luc。脂质体介导分别把质粒phPES2,CMV-Luc瞬时转染COX-2阳性的卵巢癌细胞系SKOV-3和COX-2阴性的结肠癌细胞系SW480，检测荧光素酶报告基因的表达情况。同法把质粒COX-2-BAX、pcDNA3-BAX转染SKOV-3和SW480，流式细胞仪检测细胞凋亡率。结果: 成功构建重组质粒COX-2-BAX和CMV-Luc。COX-2-Luc转染 SKOV3和SW480细胞24 h后报告基因活性分别为1554±86.5和53.7±10.9。CMV-Luc财法转染后，报告基因活性分别为9851.7±129.5和8831.0±167.3。COX-2-BAX转染SKOV-3和SW480细胞36 h后细胞凋亡率分别为10.4％，3.7％；CMV-BAX同法转染后，细胞凋亡率分别为21.7％，25.6％。结论: COX-2启动子可调控下游基因特异性地在COX-2阳性的卵巢癌细胞系中高表达，但转录效率明显低于CMV启动子。含COX-2启动子经合理修饰后可用于卵巢癌的基因治疗。

Objective:To verify that COX-2 promoter can drive its downstream genes specifically in COX-2-positive ovarian cancer cells; Moreover, comparing with CMV promoter, analyze the transcript efficiency of COX 2 promoter. Methods:Contructing the recombinant plasmids named COX-2-BAX and CMV-Luc. After transient transfection liposome-mediated with the plasmids COX-2-Luc and CMV-Luc, respectively, the expression of Luciferase reporter gene was measured in COX-2-positive ovarian cancer cell line-SKOV3 and COX-2-negative colon cancer cell line-SW480. SKOV-3 and SW480 were transfected with COX-2-BAX and CMV-BAX in the same way, respectively. The apoptosis rates were measured through flow ytometry.Results:The recombinant plasmids named COX-2-BAX and CMV-Luc were constructed successfully. The expression efficiency of reporter gene was 1554±86.5 in SKOV3 and 53.7±10.9 in SW480 after 24 hours transfected with phPES2, 9851.7±129.5 in SKOV3 and 8831.0±167.3 in SW480 after 24 hours transfected with CMV-Luc in the same way. The apoptosis rate was 10.4% in SKOV3 and 3.7% in SW480 after transfected with COX-2-BAX, 21.7%in SKOV3 and 25.6% in SW480 after 36 hours transfected with pcDNA3-BAX in the same way.Conclusions:COX-2 promoter can drive its downstream genes specifically in COX-2-positive ovarian cancer cell lines, but its expression efficiency wasmarkedly lower than CMV promoter's. With proper modification, COX-2 promoter is expected to be useful in gene therapy of ovarian cancers.