目的：选择性去除骨髓移植物中异基因反应性淋巴细胞，诱导异基因特异性低反应性。方法：用携带有FasL基因的重组腺病毒转染BALB/c小鼠来源的DC，并与C57BL/6小鼠脾淋巴细胞共培养，通过混合淋巴细胞培养等方法检测异基因特异性低反应性。结果：转染FasL基因的DC诱导了对BALB/c异基因反应性T淋巴细胞凋亡，2次混合淋巴细胞反应显著降低，但并没有抑制针对第三方的反应。结论：转染FasL基因的DC体外可有效去除骨髓移植物中异基因反应性T淋巴细胞，移植用这种方法处理过的骨髓，有希望在抑制GVHD的同时，不影响移植物抗肿瘤复发和抗感染的功能

Objective：To obtain alloantigen-specific hyporesponsivness via depletion of alloreactive cells from haematopoietic stem cell grafts.Methods：BALB/c mouse bone marrow-derived dendritic cells genetically engineered to express FasL were cultured with C57BL/6 mouse spleen lymphocytes, and MLR was performed to evaluate the alloantigen-specific hyporesponsivness.Results：FasL-transfected DC induced T cell apoptosis and inhibited alloantigen-specific hyporesponsiveness in vitro.Conclusion：DC transfected with FasL gene may prevent graft-versus-host disease by selective removal of alloreactive cells from haematopoietic stem cell grafts

全军医学科研十五计划面上项目（01MA159）