目的：研究异体的EBV-LMP2表位多肽所激活的特异性CTL对具有相同HLA-Ⅰ类分子亚型鼻咽癌细胞（CNE2）移植瘤的抑制作用，探讨异体间的免疫治疗。方法：先将CNE2细胞接种于Scid鼠背部皮下建立移植瘤模型；然后通过SSP-PCR确定CNE2的 HLA-Ⅰ类分子亚型，并选取与CNE2有相同HLA-Ⅰ类分子亚型的外周血淋巴细胞；实验再将3组不同细胞分别接种于Scid鼠北部皮下，（1）对照组：仅接种CNE2细胞；（2）T细胞组：接种未激活的淋巴细胞与CNE2混合细胞。（3）CTL组接种经EBV-LNP2多肽激活的特异性CTL与CNE2混合细胞。观察EBV LMP2多肽激活的CTL对CNE2移植瘤生长的影响。结果：CNE2于一周左右在Scid鼠背部形成肿块，病理学鉴定为低分化上皮癌；SSP-PCR显示 CNE2含有HLA-A11基因位点；含有相同基因位点的DC负载LMP2 -A11多肽所激活的CTL，明显抑制CNE2移植瘤生长。结论：EBV-LMP2多肽所激活的特异性CTL抑制HLA亚型相同的CNE2移植瘤的形成。

Objective：To investigate inhibitory effect of specific cytotoxicity lymphocytes(CTLs) pulsed by EBV-LMP2-peptide epitopes on transplantable tumor of nasopharyngeal carcinoma cell (NPC)line-CNE2 with identical HLA-Ⅰ molecule subtypes, and explore the possibility of immunotherapy among indivduals. Methods：Transplantation tumor was established through subcutaneous injection of CNE2 cells in Scid mice. HLA-Ⅰ subtypes in CNE2 cells were identified by specific site primer polymerase chain reaction (SSP-PCR). Matched HLA HLA-Ⅰmolecule subtypes with CNE2 were screened among normal volunteers. Specific CTLs stimulated by EBV-LMP2-peptide epitopes were collected for the next steps. Three groups were divided. (1) CTL group: CNE2 cells with specific CTLs stimulated by EBV-LMP2-peptide epitopes were injected into mice subcutaneousness. (2) T group: CNE2 cells with t lymphocytes were injected. (3) The control group, CNE2 cells were injected only. The results were observed in a few weeks later. Results：Transplantable tumors were observed after CNE2 having been injected into scids mice. HLA-A11 in CNE2 cells was detected by SSP-PCR. Comparing the results from the three groups, we found specific CTLs stimulated by A11-LMP2-pepetide epitopes distinctly retarded the growth of CNE2 transplantable tumor. Conclusion：Specific-LMP2 CTLs with HLA-A11 molecule subtype effectively inhibit the development of transplantable tumor of CNE2 cells with identical HLA-Ⅰ molecule subtype of three different groups were injected.

国家自然科学基金资助（基金编号为3020520），国家“973”资助项目（G1998051201），国家“863”资助项目（2001AA217091）