目的：构建MUC1肿瘤抗原表位γ1neo-PDTRP质粒，基因免疫诱导机体产生有效的体内外抗瘤效应。方法： 用Insight Ⅱ将PDTRP抗原表位与MUC1分子模拟，构建含PDTRP的抗原化抗体表达质粒γ1neo-PDTRP，体外转染检测表达。用该质粒基因免疫小鼠，免疫后ELISA动态检测血清中抗PDTRP抗体的产生及特异性细胞免疫应答；以4T1-PDTRP肿瘤细胞攻击经γ1neo-PDTRP免疫小鼠，观察免疫保护效应。结果：三串联肿瘤抗原表位PDTRPDTRPDTRP模拟了MUC1核心序列的空间结构；构建γ1neo-PDTRP质粒并在体外转染表达，基因免疫小鼠诱导出特异性抗PDTRP体液和细胞免疫应答，免疫小鼠对4T1-PDTRP肿瘤细胞的攻击具有显著的体内保护效应。结论：MUC1肿瘤抗原表位PDTRP可模拟MUC1核心序列的天然构象， γ1neo-PDTRP基因免疫在体外诱导特异性的体液和细胞免疫应答，体内诱导对机体的免疫保护效应。

Objective: To study the anti-tumor efficacy induced by antibodized tumor epitope PDTRP gene immunization. Methods:Three copies of tumor associate gene PDTRP from MUC1 tandem repeats were designed and mimicked the conformation of MUC1 by Insight Ⅱ. The γ1neo-PDTRP plasmid was further constructed, in which the PDTRP target gene was inserted into CDR3 of the γ1-neo vector. The specific humoral and cellular immune responses towards to PDTRP were detected after intraspleen immunized Balb/c mice with γ1neo-PDTRP. And the immune protection assay was also done to observe whether the mice immunized with γ1neo-PDTRP could prolong the survival after tumor challenge. Results: The conformation of three copies of PDTRP mimicked the conformation of MUC1 tandem repeats. The expression of γ1neo-PDTRP could be detected after in vitro transfect. The specific antibody against PDTRP epitope could be induced and increase to a higher titer after intraspleen injection with a γ1neo-PDTRP plasmid. And the specific proliferation and cytotoxic function of lymphocyte were also increased. There is a significant survival from mice immunized with γ1neo-PDTRP against the 4T1-PDTRP tumor challenge.Conclusions: Gene immunization with γ1neo-PDTRP could elicit both humoral and cellular tumor specific immune response and had the protective effect.

上海－联合利华研究与发展基金资助课题（2010）、国家自然科学基金面上项目（30170867）和国家重点基础研究发展计划（2001CB10006）资助课题