目的：探讨肿瘤增殖基因Ki67反义寡核苷酸（Ki67-ASODNs）对人肾癌细胞增殖及凋亡的影响。方法:将Ki67-ASODNs转染人肾癌细胞系786-0细胞，采用免疫组化、Western blot技术检测Ki67表达，细胞生长曲线、3H-TdR掺入试验等检测肾癌细胞增殖，免疫组化TUNEL法检测肾癌细胞凋亡。结果: Ki67-ASODNs处理组（10，40 μmol/L）的786-0细胞Ki67表达阳性率（%）（29.9±0.4，24.5±1.2）降低，Ki67蛋白（%）（82.1±2.2，66.6±4.2）降低，分别与对照组（33.4±0.8,100）比较,差异有显著性(P<0.01)。Ki67-ASODNs处理组的3H-TdR 掺入率（%）（44.5±4.4，34.5±3.2）与对照组（100）比较,差异有显著性(P<0.01)。Ki67-ASODNs处理组凋亡细胞阳性率（%）（12.7±0.5，23.1±2.1）增加，与对照组（9.3±2.0）比较,差异有显著性(P<0.01)。结论: 肿瘤增殖基因Ki67反义寡核苷酸能抑制人肾癌786-0细胞Ki67基因表达，进而抑制其增殖，促进其凋亡，且有剂量依赖性。

Objective:To investigate the effects of antisense oligoxydeonucleotides of Ki67 gene(Ki67-ASODNs) on the proliferation and apoptosis of human renal carcinoma cell line 786-0 cells. Methods: Human renal carcinoma cell line 786-0 cells were treated with Ki67-ASODNs (10, 40 μmol/L).The Ki67 expression of 786-0 cells was detected by immunohistochemical technique and Western blot respectively. The proliferation of 786-0 cells was studied by cell growth curves and 3H-TdR uptade assay. The apoptosis of 786-0 cells was detected by TUNEL assay. Results: The Ki67 expression rates of 786-0 cells treated by Ki67-ASODNs （29.9±0.4, 24.5±1.2）were lower than that of the control group(P<001). The Ki67 protein quantity rates of 786-0 cells treated by Ki67-ASODNs （82.1±2.2, 66.6±4.2）were reduced markedly(P<0.01). The 3H-thymidine incorporation rates of 786-0 cells treated by Ki67-ASODNs（44.5±4.4, 34.5±3.2） were lower than that of the control group(P<0.01).The apoptosis rates of 786-0 cells treated by Ki67 ASODNs（12.7±0.5, 23.1±2.1）were higher than that of the control group（9.3±2.0； P<0.01). Conclusions: Antisense oligoxydeonucleotides of Ki67 could inhibit the proliferation and induce apoptosis by blocking Ki67 expression of human renal carcinoma 786-0 cells.

江苏省卫生厅资助课题（H2000153）