目的:了解急性髓性白血病（AML）M2a细胞诱导的体外增殖T细胞的特异性杀伤作用和克隆性增殖情况。方法:利用肿瘤细胞 T淋巴细胞混合培养方法 （MLTC），分别收集3例经AML-M2a细胞体外诱导不同扩增时间的健康人T细胞，利用LDH释放方法分析其特异性细胞毒作用，同时利用RT-PCR和基因扫描方法分析经AML-M2a细胞诱导前后T细胞的24个TCR Vβ亚家族基因的表达和克隆性情况。结果：在培养第12天和第21天时，1例AML-M2a细胞诱导的3例健康人T细胞对该诱导细胞的平均杀伤率分别为39.88±7.79%和62.14±9.79%，而对AML-M2a患者和健康人外周血T细胞均无杀伤作用。 TCR Vβ谱系分析发现诱导后3例健康人T细胞均出现Vβ16的克隆性增殖。结论：AML-M2a细胞可诱导健康人T细胞成为具有克隆性增殖的特异性CTL，并以Vβ16的克隆性增殖为主。

Objective: To investigate the clonal expansion and specific cytotoxicity of TCR Vβ subfamily T cells from healthy individuals induced by acute myelogenous leukemia M2a subtype (AML-M2a) cells, respectively.Methods: T cells from three healthy individuals that were induced by AML-M2a cells by using mixed lymphocyte and tumor culture (MLTC) in different amplified stage were detected for the specific cytotoxicity by LDH release assay. The distribution and clonal expansion of TCR Vβ 24 subfamily genes of T cells before and after induction were analyzed by RT-PCR and genescan.Results: At 12th and 21st day after culture the cytotoxicity rates of three healthy individuals T cells after MLTC for the induced cells. (AML-M2a cells) were 39.88±7.79% and 62.14±9.79%, respectively, whereas there were not any cytotoxicity in the T cells of peripheral blood from the AML-M2a case and three healthy individuals. Clonal expansion of TCR Vβ16 subfamily T cells was displayed by genescan in the T cells from three donors after MLTC.Conclusions: The clonal expansion and specific cytotoxicity T cells from healthy individuals could be induced by AML-M2a cells. The clonal expansion T cells were more tended to TCR Vβ16 subfamily.

国家自然科学基金（39870358）、广东省自然科学基金（980705）、广州市科委重点科技攻关计划（98-z-03901）和广东省医学科研基金（A1999271）资助