目的：考察原核表达的重组人NDRG2蛋白对肿瘤细胞的作用。方法：通过设置不同浓度的蛋白剂量并设置对照组，采用细胞MTT比色法和细胞计数检测NDRG2蛋白对7901、HHCC细胞的抑制效果，流式细胞仪检测细胞周期的变化，体内实验采用裸鼠抑瘤试验。结果：重组人NDRG2对肿瘤细胞生长有较强的抑制作用，约50 μg/ml浓度时对肿瘤细胞生长的抑制作用明显，流式细胞术结果显示肿瘤细胞G1期变化，体外能抑制裸鼠肿瘤的生成。结论：重组人NDRG2蛋白在体内体外具有一定的抑制肿瘤细胞生长作用，在肿瘤治疗方面将具有一定的意义。

Objective: To study the effects of prokaryotically expressed recombinant human NDRG2 on tumor cell lines. MethodsRecombinant human NDRG2′s inhibitory effects on cell lines 7901 and HHCC at a range of protein concentrations were measured by MTT colorimeteric assay and cell counting against control groups. In the present study, the cell cycle changes were assessed by flow cytometry, and in vivo tumor inhibition study was performed on nude mice. Results: Recombinant human NDRG2 exerted strong inhibitory effects on tumor cell growth, and started to significantly suppress tumor cell growth at the concentration of about 50 μg/ml. Flow cytometry analysis indicated that such inhibition triggered changes to the G1 phase in the tumor cell cycle. In addition, the growth of the tumor xenografts in nude mice was signficantly suppressed in the in vivo study. Conclusion: Recombinant human NDRG2 exhibits cdertain inhibitory effects on tumor cell growth both in vivo and in vivo, which presents an interesting finding that will offers us insight into the cancer treatment.