目的：观察顺铂作用下K562细胞及白血病骨髓单个核细胞（BMMNC）的细胞周期变化和转染Chk1/2反义寡核苷酸对顺铂诱导下K562细胞及白血病BMMNC凋亡的影响。方法：用流式细胞仪检测顺铂作用下K562细胞及白血病BMMNC的细胞周期变化。转染Chk1和Chk2反义寡核苷酸于K562细胞及白血病BMMNC，检测顺铂作用下转染细胞的凋亡率。结果：10 μmol/L顺铂作用下K562细胞和白血病BMMNC均出现S期阻滞，转染Chk1/2反义寡核苷酸可明显增加顺铂诱导下K562细胞凋亡，转染Chk1反义寡核苷酸可明显增加顺铂诱导下白血病BMMNC的凋亡率，但转染Chk2反义寡核苷酸未增加白血病BMMNC的凋亡率。结论：Chk1在肿瘤细胞凋亡中有重要调节作用，可作为肿瘤增敏治疗的有效靶点，Chk2在肿瘤细胞凋亡中的作用需进一步研究。

Objective: To investigate the cell cycle change of K562 cells and bone marrow mononucleate cells(BMMNC) of leukemic patient induced by DDP and the role of antisense oligonucleotide targeting Chk1/2.Methods: The change of cell cycle was assayed by means of flow cytometer after different interval in which the K562 cells and BMMNC of leukemia are treated by DDP. K562 cells or BMMNC are transfected with the antisense oligonucleotide complementary to Chk1/2 by lipofection. The apoptosis of K562 cells and BMMNC induced by DDP was investigated by flow cytometer after transfection. Results: K562 cells and BMMNC of leukemia arrested at S phase at 10μmol/L of DDP. The antisense oligonucleotide targeting Chk1 or Chk2 could increase the apoptosis of K562 cells treated with DDP. The antisense oligonucleotide targeting Chk1 could increase the apoptosis of leukemic BMMNC treated with DDP, but the antisense oligonucleotide targeting Chk2 could not increase the apoptosis of BMMNC of leukemia induced by DDP.Conclusion: Chk1 may take part in apoptosis regulation of leukemia cells and be used as target of leukemia therapy.

国家自然科学基金（30271472）