目的: 研究fat-1基因在人乳腺癌细胞内的表达、功能及其对乳腺癌细胞增殖的影响。方法: 把fat-1 基因插入到腺病毒的穿梭载体中，与骨架载体同源重组，构建腺病毒重组载体 (Ad.GFP.fat1)，将通过包装细胞系（293）产生的腺病毒感染人乳腺癌株QMR2细胞。提取细胞的总RNA，以fat-1的反义mRNA 作探针，用Northern Blot检测fat-1 基因在人乳腺癌株QMR2细胞内的表达。流式细胞仪分析n-3脂肪酸脱氢酶对人乳腺癌株QMR2细胞增殖的影响。气象色谱仪分析n-3脂肪酸脱氢酶对人乳腺癌株QMR2细胞的n-6 PUFAs/n-3 PUFAs含量影响。结果: fat-1 基因在人乳腺癌株QMR2细胞 中能有效异源表达，2 d后检测到fat-1mRNA的条带。fat 1基因抑制了人乳腺癌株QMR2细胞的增殖，降低了20%（P<0.05）；同时降低了人乳腺癌株QMR2细胞n-6 PUFAs/n-3 PUFAs含量降低。结论: 腺病毒介导的fat-1 基因能在人乳腺癌株QMR2细胞内有效异源表达，且抑制人乳腺癌株QMR2细胞的增殖

Objective: To transfer the gene of n-3 fatty acid desaturase fat-1 into human breast cancer cell QMR2 by adenovirus vector and study the effect of the gene on proliferation of QMR2 cells. Methods: The gene fat-1 was cloned into the shuttle vector of adenovirus, and homologously recombined with an adenoviral backbone vector (pAdEasy 1) to generate the recombinant adenovirus Ad.GFP.fat1; the virus was packaged in 293 cells, inoculated on the breast cancer cells QMR2; total RNA of the cells was hybridized with antisense RNA of fat-1 mRNA by Northern to analyze the expression of fat-1; the effect of fat-1 on the proliferation of QMR2 cells was analyzed by Flow Cytometry; the content of n-6 PUFAs/n-3 PUFAs was analyzed by Gas Chromatography.Results:The high titer recombinant virus was got through DNA recombinant; the fat-1 mRNA appeared in breast cancer cell QMR2 after virus Ad.GFP. fat1 infected the cells for 2 days; compared with the control cells (Ad.GFP), proliferation of QMR2 cells was inhibited by the gene fat-1, decreased by 31%(P<0.05); moreover, fat-1 gene decreased content of n-6 PUFAs/n-3 PUFAs. Conclusion: The gene fat-1 was heterologously expressed in human breast cancer cell QMR2 via adenovirus, and the expression produced an inhibitory effect on proliferation of the cells.

R730.2 R735.2

国家自然科学基金（No.30271217）;青岛市科技局科技计划项目（No.03-1-YN-14）