目的：应用血清蛋白组学技术优化肿瘤抗原筛选方法，筛选鼻咽癌肿瘤抗原。方法：以免疫印迹技术筛选出含有特异性抗体的鼻咽癌患者血清，运用免疫沉淀技术对血清与鼻咽癌细胞株（CNE1）的裂解物进行处理以富集肿瘤抗原，正常人血清做对照，经凝胶电泳找出差异条带，通过质谱分析、数据库搜索和信息学分析鉴定差异蛋白。结果：应用此方法找出存在于鼻咽癌样本中的明显差异条带1条，重复性较好，经鉴定该条带为膜结合IgM。结论：采用上述方法筛选肿瘤抗原行之有效，可能成为肿瘤抗原筛选的一种简单、便捷、灵敏的有效方法，得到鼻咽癌候选抗原膜结合IgM。

Objective: To screen tumor antigens of nasopharyngeal carcinoma by serological proteomics technologies. Methods:The nasopharyngeal carcinoma cell line CNE-1 was used as a source of tumor cell proteins for Western blot analysis in which individual sera with special immunoreaction were selected and for immunoprecipitation analysis. Then, differential proteins are analyzed by mass spectrometry and bioinformatics methods.Results: Membrane-bound IgM was identified in the differential protein band. Conclusion: It may be a convenient, simple and sensitive way to screen tumor antigens by proteomics technologies. Membrane-bound IgM may probably be a novel antigen of nasopharyngeal carcinoma.

国家自然科学基金面上项目（No.30270524）