目的: 金黄色葡萄球菌肠毒素A作为一种超抗原，其抑瘤活性一直受到关注，其直接应用于肿瘤治疗的报道较少，本研究对其抑瘤活性进行了分析。方法: 利用亲和层析纯化出重组SEA，注射接种了黑色素瘤的小鼠，观察其抑瘤效应。结果: 各组剂量的重组SEA均能有效抑制肿瘤的生长，其高、中、低剂量重组SEA的抑瘤率分别为79.3%、75.6%和73.8%，而原位注射（中剂量）的抑瘤率为90.6%。治疗小鼠的肿瘤组织出现大量的T细胞浸润；此外，SEA以免疫的形式刺激动物，能明显产生特异性抗体，并在一定程度上增强小鼠对肿瘤转移的抵抗力。结论: 重组SEA对小鼠黑色素瘤的能产生明显的抑制效应，尤其是原位注射，这为SEA的靶向治疗肿瘤奠定了基础

Objective: To evaluate the antitumor activity of recombinant SEA for therapy of B16 melanoma established in C57BL/6 mice. Methods: C57BL/6 mice with melanoma were treated with the purified rSEA. The tumors were isolated and weighted. Results: Tumor growth was apparently inhibited by rSEA at high, middle, and low doses intraperitoneally, whose inhibition ratio were 79.3%, 75.6 % and 73.8% respectively. rSEA treatment in situ could inhibit tumor growth more effectively(90.6%). Further study showed that numerous CD8+ and CD4+ T cell were infiltrated in tumor tissues, which were consistent with tumor growth inhibition induced by rSEA. Conclusions: rSEA could inhibit tumor growth effectively, especially the treatment in situ. This study paves the way for tumor immunotherapy with targeted SEA.

国家自然基金资助课题（30300417）