目的：探讨CpG基序在人食管癌Eca-109细胞疫苗研究中的佐剂效应。方法：以Eca-109细胞膜表面小肽刺激DC作为食管癌疫苗，HL-60细胞膜表面小肽刺激DC作为对照疫苗。以CpG基序作为佐剂，以CBPw作为对照佐剂，测定各组的T细胞增殖试验和CTL活性。结果： 佐剂组的T细胞活性明显高于其余各组（P＜0.01）； 人食管癌疫苗组的T细胞活性明显高于其余各组（P＜0.01）； 与Eca-109细胞匹配的HLA也影响了T细胞活性的发挥。结论：CpG基序是人食管癌疫苗的有效佐剂，HLA对T细胞功能有影响。

Objective:To investigate the adjuvant effects of CpG motif-containing oligodeoxynucleotide (CpG) in vitro on human esophageal cancer vaccine.Methods:Matured dendritic cells (DC) activated by peptides on Eca-109 cell membranes were used for human esophageal tumor vaccine and matured DC activated by peptides on HL-60 cell membranes were used for control vaccine. With adjuvant of CpG or the cell wall skeleton of coryne bacterium parvum (CBPw), we analyzed each group's T-cell proliferation and cytotoxic T lymphocyte (CTL) response. Results:The adjuvant of CpG or CBPw markedly enhanced T-cell proliferation and CTL response（P＜0.01）; The groups with human esophageal tumor vaccine had markedly greater T-cell proliferation and CTL response than other groups（P＜0.05）; The HLA gene locus alleles to match Eca-109 cell gene affected the activity of T cells.Conclusion:CpG may be the optimal adjuvant of human esophageal tumor vaccine and the HLA gene locus may affect T cells' function.

2002教育部科学技术重点项目; 2002河南省重点科技攻关项目(0223033900); 2003年河南省科技厅攻关项目(324410035)