目的：探讨JNK/SAPKs信号转导通路在榄香烯抗肝癌效应中的作用，为阐明榄香烯抗癌效应的分子机制提供参考。方法：气相色谱法检测榄香烯在Hca-F肝癌细胞内的分布。透射电镜观察SMMC7721凋亡细胞的超微结构变化。利用Western-blotting对榄香烯处理的HepG2肝癌细胞JNK/SAPKs进行的活性分析。利用RT-PCR检测榄香烯处理的SMMC7721肝癌细胞的DAXX基因表达。结果：榄香烯标准品在8.42 min时出现洗脱峰。榄香烯处理3 h后，SMMC7721细胞开始发生具有凋亡特征的变化。榄香烯处理组HepG2细胞的JNK/SAPKs激酶活性次于热休克处理组，高于对照组。榄香烯处理的SMMC7721细胞组未见DAXX基因的表达。结论：榄香烯能够进入细胞内。其引起的肿瘤细胞凋亡可能是通过活化JNK/SAPKs来诱导的。DAXX传入途径在榄香烯诱导的JNK/SAPKs活化过程中可能不起主要作用

Objective:To find out the role of JNK/SAPK signaling-transduction pathway in the effect of elemene against hepatocarcinoma, offering the clue to ilustrate the molecular mechanisms of antitumor effects of elemene.Methods:The detection of the distribution of elemene in Hca-F cells was detected by gas chomatography and apoptotic changes in elemene treated. SMMC7721 cells were examined by TEM. After elemene treatment, the activation of JNK/SAPK in HepG2 cellls and the DAXX gene expression in SMMC7721 cells were detected by Western blotting and RT-PCR respectively.Results:Gas chomatography showed that elemene was detected at 8.42 minute. The SMMMC7721 cells treated by elemene for 3 hours began to show typical apoptotic changes . The JNK/SAPK activity in HepG2 cell treated with heat shock was the highest of all groups and the group treated with elemene was the next and the control group is the lowest one. There was no DAXX gene expression in SMMC7721 cells treated with elemene. Conclusion:Elemene can diffuse into cells. Tumor cell apoptosis treated with elemene may be induced by JNK/SAPK activating and DAXX signal pathway may not play key role in JNK/SAPK activation induced by elemene.

国家自然科学基金重点项目资助(39730440-Ⅱ);辽宁省普通高等学校中青年学科带头人辽教办发\[200144\]号; 优秀青年骨干教师选拔工程