目的：从机体的免疫应答来探讨甲型流感病毒对小鼠S180腹水瘤的作用机制。方法：小鼠S180腹水瘤模型建立后，分别腹腔注射生理盐水或甲型流感病毒治疗15 d。观察实验小鼠的生存时间；用ELISA法检测小鼠血清IL-2, IL-6和TNF-α的含量； DNA ladder法、流式细胞仪（FCM）、荧光显微镜检查和电镜（EM）用以检测腹水瘤细胞凋亡。结果：用甲型流感病毒治疗的实验组荷瘤小鼠的平均存活时间、生存率均显著高于生理盐水对照组。实验组小鼠血清中IL-2, IL-6, TNF-α的含量亦显著高于对照组。实验组小鼠腹水瘤细胞DNA发生了特异性降解，出现典型的DNA 梯形条带。结论： 甲型流感病毒对于小鼠S180腹水瘤具有治疗作用，在此过程中，有机体免疫系统的参与和肿瘤细胞凋亡的产生，而病毒本身通过腹腔注射并不引起小鼠的死亡

Objective:To investigate the immunological mechanism of influenza A virus for murine S180 ascites sarcoma.Methods:After inocutation with S180 sarcoma cells, mice were i.p. injected with influenza A virus or vehicle 15 days. The average living time and survival rate of the mice were examined. The levels of IL-2, IL-6 and TNF-α were detected. The sarcoma cell′s apoptosis was detected by DNA ladder, flow cytometry (FMC), fluorescent microscope and electron microscope (EM). Results: The average living time and survival rate of the mice injected with Influenza A virus were significantly longer or higher than that of the controls. The levels of IL-2, IL-6 and TNF-α also had the same differences. The apoptosis cells were detected by EM and fluorescent microscope. Sub-diploid peaks were observed by FCM analysis and DNA ladder was seen after electrophoresis in the ascites cells. Conclusion:Our results demonstrated that the feasibility and potential of delivery of influenza A virus as a general means for the treatment of S180 ascites sarcoma.

国家自然科学基金资助项目(39970830）