目的：研究67 kD层黏连蛋白受体（67kD LN-R）反义寡核苷酸(antisense oligonucleotide, ASODN)对卵巢癌细胞HRA体外侵袭转移能力的影响。方法:采用流式细胞仪、RT- PCR及Transwell小室的方法检测反义核酸对67 kD LN-R基因和蛋白表达影响，及转染前后细胞体外侵袭转移能力变化。结果: 67 kD LN-R ASODN可从蛋白质和mRNA水平下调67 kD LN-R的表达，且下调作用呈剂量依赖性。与正义组及对照组相比差异有显著性（P<0.05）。体外侵袭实验证实转染后HRA穿透人工基底膜的能力显著降低并呈现剂量依赖性。结论: 67 kD LN-R ASODN可降卵巢癌细胞体外侵袭转移能力，有望为卵巢癌治疗提供新方向。

Objective:To investigate the effect of 67 kD laminin receptor antisense oligonucleotides (67kD LN-R ASODN) on the invasion and metastasis abilities of ovarian carcinoma cell line HRA.Methods:The ASODN of 67 kD laminin receptor and its control, sense oligonucleotides (SODN) were synthesized and transfected into the target cells. RT-PCR and FCM methods were performed to evaluate the 67 kD LN-R gene and protein expression levels to identify the efficiency of ASODN. The invasiveness of transfected cells was measured quantitatively by matrigel invasion assays (transwell chamber). Results: The 67 kD LN-R gene and protein expression and invasiveness of HRA cells treated with ASODN of different final concentration were significantly decreased compared with that transfected with SODN and the controls（P<0.05）. Conclusion:67 kD LN-R ASODN has a significant inhibitory effect on the invasiveness of human ovarian carcinoma cell line HRA in a dose-dependent manner. It may become a new gene therapeutic agent for ovarian carcioma.

山东省科技厅2004年度国际科技合作计划资助课题