目的：研究尿路斑块蛋白Ib启动子启动Smac表达的膀胱癌特异性及活性。方法： 采用RT-PCR和免疫组化方法分别检测膀胱癌细胞株BIU-87和其他多种非膀胱癌细胞株在转染含尿路斑块蛋白Ib启动子和Smac基因的真核表达质粒pcDNA3-UpIb promoter-Smac前后Smac mRNA和蛋白质的表达变化。结果：BIU-87在转染后Smac mRNA的表达比转染前增强约2.1倍，Smac蛋白表达阳性细胞率也由转染前的约25.6%增加为转染后的约70.5%；非膀胱癌细胞株转染前后Smac mRNA及蛋白的表达没有显著性变化。结论：尿路斑块蛋白Ib启动子启动Smac表达具有膀胱癌靶向性及相当启动活性，为膀胱癌的靶向基因治疗研究奠定了基础。

Objective: To investigate the bladder cancer-specificity and activity of the expression of Smac gene promoted by UpIb promoter. Methods:Smac mRNA and protein were detected in BIU87 cell line and many other non-bladder cancer cell lines before and after transfection with pcDNA3-UpIb promoter-Smac including UpIb promoter and Smac gene by RT-PCR and immunohistochemical method, respectively.Results:The expression of Smac mRNA in BIU87 cell line increased about 2.1 times after transfection . The Smac protein positive cell rate of BIU-87 cell line was about 25.6% before transfection and it increased to about 70.5% after transfection. There was no significant difference about the expression of Smac mRNA and protein in non-bladder cancer cell lines before and after transfection. Conclusions: UpIb promoter could promote the expression of Smac gene with bladder cancer-specificity and considerable activity, which laid the foundation of target gene therapy for bladder cancer.

国家自然科学基金（基金编号30271301）