目的：探讨脂氧合酶抑制剂NDGA在体外对结肠癌HT-29细胞株生长抑制、诱导凋亡，并对端粒酶表达的影响进行研究。方法： 应用MTT法绘制生长曲线，倒置相差显微镜观察细胞形态变化；扫描电镜观察细胞超微结构变化，观察凋亡小体； RT- PCR检测端粒酶催化活性亚单位（hTERT）表达水平变化。结果： 不同浓度NDGA处理HT-29结肠癌细胞，随着药物浓度加大，细胞形态变圆，体积缩小，从瓶壁上逐步脱落，肿瘤细胞生长抑制。应用扫描电镜可见凋亡小体形成。对照组结肠癌HT-29细胞hTERT mRNA呈阳性表达，随着药物浓度上升， hTERT mRNA表达水平逐步下降。结论： NDGA对结肠癌HT 29细胞具有抑制生长、诱导凋亡作用，并且具有剂量依赖效应。端粒酶参与其诱导凋亡的过程。

Objective: To investigate the effect of NDGA, the lipoxygenase inhibitor, on colon cancer cell line HT- 29 in vitro from the aspects of cell growth inhibition, cell apoptosis and its impact on the expression of telomerase. Methods: We applied respectively i) MTT to draw the growth curve. ii) inverted phase contrast microscope to observe morphologic change of cells, iii) scanning electron microscope to observe changes of cell′s ultra- microstructure and apoptotic body. iv) RT-PCR to detect the changes of the expression of human telomerase reverse transeriptase (hTERT).Results:Different concentrations of NDGA were used to dispose cancer cells separately, with the rise of the drugis concentration, the form of cells became round, the volume waned, cells abscised from the inner surface of the bottle. and growth inhibition became increasing abvious. Also through scaming electron microscope, apoptic bodies could be found colon cancer cell line HT- 29 showed positive expression of hTERTmRNA, which became weaker following the rising of the drug's concentration. Conclusions: NDGA which, displays the relying effect of doses, can inhibit the growth of colon cancer cell line HT- 29 and induce its apoptosis, telomerase plays an active role in this course.