目的：探讨HSPgp96对小鼠PEM体外抗肿瘤细胞毒作用的影响。方法： 分离巯基乙酸盐诱导的小鼠PEM，随机分为培养液对照组、LPS诱导组、HSPgp96诱导组。应用硝酸还原酶法、MTT法、扫描电镜分别观测PEMNO的生成、对肝癌细胞H22的细胞毒作用及形态学变化。结果： 在HSPgp96的体外诱导下，小鼠PEMNO的生成量显著增加，对肝癌细胞H22的细胞毒作用明显增强，其效应与LPS相当。结论： HSPgp96可在体外有效激活PEM的抗肿瘤细胞毒作用，NO是PEM杀瘤机制中的重要效应分子之一。

Objective: To evaluate the cytotoxic effect of PEM induced by HSPgp96 on anti-tumor in vitro. Methods:PEM separated from mice induced by thioglycolate were divided into three groups randomly: Culture medium in control; LPS-induced group; HSPgp96-induced group. The production of NO, the cytotoxic effect to H22 cells and the morphologic change of PEM were investigated separately by enzyme method, MTT assay and scanning electron microscope. Results:In vitro, HSPgp96 can increased NO production from PEM of mice and significantly enhance the cytotoxic effect of PEM to H22 cells as well as LPS. Conclusion:HSPgp96 can effectively induce the cytotoxic effect of PEM on anti-tumor in which NO is one of the capital effective molecules in vitro.

江苏省教育厅基金资助项目（03KJD320183）