目的：探讨血管内皮生长因子(vascular endothelial growth factor,VEGF)及其受体(KDR)在前列腺癌细胞中的表达及意义。方法：免疫细胞化学和Western blot检测体外培养的LNCaP,PC-3,PC-3M,DU-145和22RV1前列腺癌细胞中VEGF及其受体KDR的蛋白表达，RT-PCR检测mRNA，ELISA检测前列腺癌细胞培养上清VEGF蛋白含量。结果：免疫细胞化学染色显示VEGF和KDR在前列腺癌细胞LNCaP, PC-3, PC-3M, DU-145和22RV1中均有表达，但表达水平略有差别。LNCaP, PC-3和DU-145细胞中VEGF和KDR蛋白表达及其mRNA的含量显著高于PC-3M和22RV1(P＜0.01)。细胞培养上清中VEGF蛋白含量结果与免疫细胞化学染色结果相同。结论: VEGF及其受体KDR在前列腺癌细胞中的表达量不近相同，但二者的表达对研究前列腺癌的发生发展及其机理有重要意义。

Objective:To investigate the expression of VEGF and its receptor (KDR) in prostate cancer cell lines. Methods: Immunocytochemical staining and Western blot and reverse transcriptase-polymerase chain reaction(RT-PCR)were employed to test the expression of VEGF and its receptor KDR in five human prostate cancer cell lines, LNCaP, PC-3, PC-3M, DU-145, 22-RV1. ELISA was employed to test VEGF concentration in culture supernatant of prostate cancer cell lines. Results:All five prostate cancer cell lines expressed VEGF and KDR. The expression of protein and mRNA in PC-3, DU-145 and LNCaP cell lines was higher than that in PC-3M and 22RV1 cell lines (P< 0. 01). The result of the VEGF concentration in cell culture supernatants is as same as that of immunocytochemical staining.Conclusion: Although they were expressed differentially, both VEGF and KDR were present in prostate cancer cell lines. The expression of VEGF and KDR might be related with the pathogenesis and progress of prostate cancer.

国家863计划资助项目(2001AA215321)