目的： 制备和保存满足临床造血干细胞移植要求的脐带血造血干细胞。方法：按标准操作规程（SOP）进行脐带血的采集、分离、冷冻保存和检测。结果：在采集的6 232份脐带血中，采集的净血量、TNC、CD34+ 细胞百分率和CFU-C产率均值分别为90.7±22.2 ml, 10.5±3.4×108, 0.24±0.15%, 78.9±44.14/105 NC。已保存符合标准的脐带血4 500份；分离后TNC回收率超过80％，而CD34+细胞、CFU C回收率则超过90％，细胞活率无明显改变；冻存融化后细胞回收率80％～110％，细胞活率>90％。在接受569例无血缘关系患者查询中，93%患者可查找到≥4个HLA-A, B和DRB1位点相合的脐带血。结论：可为临床造血干细胞移植提供合格的脐带血造血干细胞。

Objective:To prepare and cryopreserve umbilical cord blood(UCB) units for clinical hematopoietic stem cell transplantation. Methods:HSC of UCB units that met our standards were collected, separated cryopreserved and tested. Results:4 500 (72.2%) of 6 232 cord blood units were suitable for preservation. Average net volume, numbers of total nucleated cells, percentage of CD34+ cells and CFU-C of the UCB units were 90.7±22.2 ml, 10.5±3.4×108, 024±0.15%, 78.9±44.14/105 NC, respectively. After separation, the cell recovery of nucleated cells, CD34+ cells and CFU-C were 82.1±6.52%, 92.5±6.15% and 90±9.67%, respectively. The cell viability after separating was over 95%.After thawing, the recovery of nucleated cells, CD34+ cells and CFU-C were 84.2±10.1%, 96.5±21.8% and 115.1±23.3%, respectively. The cell viability was 93.8±4.4%. Among the 569 searching cases , 532 (93%) cases could found 4/6 of HLA-A, B and DR loci matched UCB units in our bank, including 39 (6.58%) found 6 loci matched and 237 (41.65%) found 5 loci matched. Conclusion:These results indicated that we can supply high quality cord blood HSC for clinical transplantation.

美国纽约中华医学基金（CMB）（1－748）； 天津市社会发展计划重大项目（003119811）