目的：从真菌松萝中提取地衣酸并研究地衣酸对体外培养前列腺癌PC-3M细胞增殖的抑制效应。方法：用细胞培养、细胞生长抑制实验（MTT法）观察PC-3M细胞形态、细胞生长密度和检测细胞生长抑制率，用3H-TdR掺入法测定细胞增殖过程中DNA的含量。结果：与对照组相比，实验组PC-3M细胞形态异常、细胞生长密度降低，细胞生长抑制率增强，3H-TdR掺入量明显减少，并呈剂量依赖关系，与地衣酸浓度的相关系数分别为0.9799与-0.9525。结论：地衣酸对体外培养PC-3M细胞具有抑制效应。

Objective:To extract Usnic acid from usnea longisima ach, and to evaluate the ability of Usnic acid to inhibit growth of human prostate cancer cells PC-3M in vitro. Methods:The cells were observed by microscope.The cytotoxicity to cancer cells was determined by MTT assay. DNA synthesis was tested by 3H-TdR in corporation assay in the experiments. Results: There were significant differences between PC-3M cells lest group and control group. The Usnic acid had streng thened cytotoxicity, and the rate of 3H-TdR incorporation was lower than that of the control in a dose-dependent manner (r=0.9799, r=-0.9525). Conclusion: The results suggested that the Usnic acid has inhibitory effects on growth of human PC-3M cells in vitro.

日本国际协力事业团（JI-CA）资助专项技术合作项目第59项