研究Ras homology C(RhoC)在胃癌转移中的作用。方法:利用Vector NTI软件设计并构建RhoC 的小干扰RNA(siRNA)载体,利用脂质体介导法将一组RhoC siRNA分别转染胃癌SGC7901细胞。筛选出稳定抗性克隆;Western blot检测RhoC siRNA转染后的抑制效应,Cell Counting Kit-8检测RhoC siRNA转染细胞株的生长速度;MTT法检测转染细胞株对化疗药物的敏感性;损伤刮擦实验和TRANSWELL小室实验分别检测转染细胞株的迁移与侵袭能力。结果:在计算机辅助下成功地设计并构建了5个RhoC的小干扰RNA载体,Western blot显示其中一个RhoC的小干扰RNA RhoC-s362对 RhoC的表达有明显的抑制作用;尽管下调RhoC的表达对胃癌细胞的生长和药物敏感性无明显影响,但可显著抑制肿瘤细胞的迁移与侵袭。结论:RhoC siRNA RhoC-s362能够明显抑制胃癌SGC7901细胞中RhoC的表达,并进而抑制胃癌细胞的迁移与侵袭,提示RhoC可能在胃癌转移中发挥重要作用。

To study the effect of RhoC on the metastasis of gastric cancer cells. Methods: A group of RhoC -specific small interfering RNAs (siHNA) were designed and the related vectors were constructed with the assistance of computer. The vectors were transfected into human gastric cancer cell SGC7901 by Iipofectamine2000. The protein expression of RhoC in the translectants was examined by Western blot.Cell growth rate was determined will a Cell Counting Kit -8. MTT assay was used to evaluate the sensitivity of the transfectants to anticancer drugs. Wound-healing and invasion assays were used to examine the abilities of migration and invasion, respectively. Results:Five RhoC-specif-ic siRNAs were designed and the related vectors were constructed successfully. Western blot showed that RhoC-specific siRNA, RhoC-s362, dramatically inhibited RhoC expression. Though down-regulation of RhoC expression did not affect the proliferation and chemo-sensitivity of the transfectants; it did suppress the migrating and invasive abilities of gastric cancer cells. Conclusion: RhoC siRNA RhoC-s362 can effectively inhibit RhoC expression and restrain the migration and invasion of the SGC7901 , suggesting that RhoC play an important role in the metastasis of gastric cancer.

国家自然科学基金项目（N0：30400535）