目的： 探讨应用RNA干扰技术沉默Aurora-A基因表达，研究其对人卵巢癌SKOV3细胞增殖的抑制作用。方法： 设计合成两对特异性针对Aurora-A基因的Oligo siRNA，转染至SKOV3细胞中，采用RT-PCR和Western blot检测Aurora-A mRNA和蛋白表达情况，同时利用MTT试验和流式细胞仪观察转染后细胞增殖抑制和凋亡情况。结果：转染Oligo siRNA后，SKOV3细胞Aurora-A mRNA表达受抑制(P<0.01)，蛋白表达水平降低;细胞增殖的抑制率和细胞凋亡率明显增高(P<005, P<0.01)。结论： 体外合成的特异性针对Aurora-A基因的Oligo siRNA对卵巢癌细胞株SKOV3中Aurora-A基因表达和细胞增殖均有明显抑制作用，为进一步研究Aurora-a基因的功能提供了实验基础。

Objective: To investigate the inhibitory effect of RNA interference (RNAi) on the expression of Aurora-A in SKOV3 cells and on proliferation of SKOV3 cells. Methods: Two pairs of oligo small interference RNA (Oligo siRNA) specific for Aurora-A were designed for RNAi and were transferred into SKOV3 cells. The expression of Aurora-A were detected by RT-PCR and Western blot. Furthermore, the cell proliferation and apoptosis were observed by MTT and FCM after transfection. Results:After transfection with Oligo siRNA, mRNA and protein level of Aurora-A gene in SKOV3 cells were obviously reduced, while the inhibitory rate of proliferation and apoptosis rate in SKOV3 cells were increased significantly. Conclusion: The Oligo siRNA specific for Aurora-A can reduce the expression of Aurora-A gene and induce apoptosis of SKOV3 cells.