目的: 观察MHC半相合脾加骨髓细胞移植抗小鼠H22实体瘤的效果。方法: 以皮下接种H22肝癌细胞的BALB/c×C57BL/6杂交F1代雌性小鼠为受鼠，以健康雌性F1、雄性C57BL/6、雄性C3H小鼠为MHC全相合、半相合、不相合供鼠，观察移植后的抑瘤情况；观察供鼠细胞经60Co照射的MHC半相合移植对WBC、生化和嵌合体的影响；比较供鼠细胞经与不经60Co照射的MHC半相合移植的GVHD情况。结果: 供鼠细胞经/不经60Co照射的MHC半相合移植小鼠的肿瘤明显较小，与单纯化疗未进行移植者比较，差异具有统计学意义（P<0.05）；但受鼠未经化疗预处理的MHC半相合细胞输注没有出现抗肿瘤效应；供鼠细胞经7.5 Gy 60Co照射的MHC半相合移植能明显降低GVHD反应,且对外周血白细胞、生化无不良影响。结论: 经7.5 Gy 60Co照射的MHC半相合脾加骨髓细胞移植能对H22肝癌细胞产生移植物抗肿瘤效应并降低GVHD反应。

Objective: To observe the anti-tumor effects of transplanting MHC half-matched bone marrow cells combined with spleen cells in a murine model bearing H22 solid tumor. Methods: Female (BALB/c×C57BL/6) F1 mice (CB6F1，H-2Kb/d) were subcutaneously inoculated with H22 cells to develop a solid tumor model; the model mice were taken as recipients. The syngenic, half-matched, and mismatched donor bone marrow cells were from healthy female F1, male C57BL/6(H-2Kb), and male C3H(H-2K) mice, respectively. The tumor weights were measured 18 days after transplantation. The WBC, biochemistry parameters and the formation of chimerism were observed in mice transplanted with MHC half-matched donor cells irradiated by 7.5Gy 60Co. Meanwhile, graft-versus-host disease (GVHD) was assessed and the results were compared between mice transplanted with radiated and non-radiated donor cells. Results: The tumor weights of mice transplanted with donor cells (radiated/non-irradiated by 60Co) were significantly decreased compared with those of the mice receiving chemotherapy alone（P<0.05）. No anti-tumor effect was found to be induced by MHC half-matched cells in mice receiving no pretreatment of chemotherapy. The transplantation of MHC half-matched donor cells radiated by 7.5 Gy 60Co obviously attenuated GVHD and had no adverse effects on peripheral WBC and functions of liver and kidney. Conclusion:Transplantation of 7.5 Gy 60Co irradiated, MHC half-matched bone marrow cells combined with spleen cells can induce a graft-versus-tumor effect in mice bearing H22 tumor and can attenuate GVHD.

福建省自然科学基金(C0310035); 福建省医学创新课题(2003-CX-16)