目的: 探讨不同肿瘤细胞系柯萨奇病毒 腺病毒受体（CAR）和整合素的表达水平与5型腺病毒感染效率的关系，为腺病毒基因治疗研究奠定基础。方法: 利用瞬时转染CAR的真核表达质粒提高肿瘤细胞表面CAR的表达，应用抗体封闭细胞表面的CAR和整合素后，通过流式细胞仪和荧光素酶分析法测定腺病毒Ad5-CMV-EGFP和Ad5-CMV-Luc对肿瘤细胞的感染效率和基因表达水平的变化。结果： 不同肿瘤细胞表面CAR和整合素的表达水平是不同的，其中SMMC-7721和A549细胞CAR的表达量最高，而K562细胞CAR的表达水平最低；荧光显微镜和流式细胞仪检测Ad5-CMV-EGFP对肿瘤细胞的感染效率，结果显示5型腺病毒对于SMMC-7721和A549细胞的感染效率最高，而对于K562细胞则很低；瞬时转染表达CAR的真核质粒可提高多种肿瘤细胞表面CAR的表达，较大幅度提高了5型腺病毒的感染效率。而抗体封闭肿瘤细胞表面的CAR或整合素后，腺病毒感染效率显著下降。结论:肿瘤细胞表面CAR和整合素的表达水平决定了腺病毒对肿瘤细胞的感染效率。

Objective: To investigate the relationship between adenovirus type 5 infection efficiency and expression of Coxsackie virus adenovirus receptor (CAR) and integrin in different tumor cell lines, providing a basis for further study of adenovirus gene therapy. Methods: The expression levels of CAR in different cell lines were examined by flow cytometry. The cells were infected with Ad5-CMV-GFP and Ad5-CMV-luc carrying report genes, tested the infection efficiency and determined their expression levels by flow cytometry and Luciferase Assay. The variation of adenovirus infection efficiency was evaluated and compared when the CAR expression was enhanced on cell surface with plasmid expressing CAR or blocked by antibodies of CAR and integrin. Results: The highest CAR expression was found in SMMC-7721 and A549 cell lines, the lowest in K562 cells. The results of fluorescence microscope and flow cytometry suggested that Ad5 had the highest efficiency in infecting SMMC-7721 and A549 cell lines, and the lowest in infecting K562 cells. Conclusion：The infection efficiency of adenovirus is determined by the levels of CAR and integrin on cancer cells.

国家重大基础研究发展规划(“973”计划)项目（NO.2004CB518800