目的： 探讨腺病毒介导乙型肝炎病毒表面抗原（AdVHBsAg）基因修饰树突状细胞（dendritic cell,DC）瘤苗体外生物学活性。方法：将腺病毒表达载体AdVHBsAg转染人单个核细胞来源的DC，构建AdVHBsAg-DC肝癌瘤苗，采用Western blotting法鉴定转染基因表达，FACS检测表面分子和内吞功能，3H-TdR法检测T细胞增殖反应的能力，MTT法检测CTL活性。结果：HBsAg基因转染后，Western blotting法检测结果示HBsAg基因表达于转染的DC，表明腺病毒介导的HBsAg基因转染的有效性。AdVHBsAg-DC可高表达CD1a、CD11c、 CD83、CD86和HLA-DR，但内吞功能较DC组降低(P<0.05)。AdVHBsAg- DC刺激自体T细胞增殖功能均明显高于DC对照组和AdVLacZ-DC组(P<0.05)。AdVHBsAg- DC体外诱导CTL对HepG2215肿瘤细胞的杀伤作用具有特异性。结论：肝癌相关基因HBsAg可作为乙型肝炎病毒相关性肝癌的切入点，该研究为HBV相关肝癌DC体内免疫治疗提供了实验依据。

Objective： To study the biological characteristics of HBsAg gene-modified DC tumor vaccine in vitro. Methods: Recombinant adenovirus expression plasmid AdVHBsAg was transfected into human monocyte-derived dendritic cells to construct AdVHBsAg hepatocarcinoma tumor vaccine. The expression of transfected gene was detected by Western blotting. Surface molecules and phagocytosis of AdVHBsAg-DCs were detected by FACS. Autologous T cell proliferation stimulated by AdVHBsAg-DCs was detected by 3H-TdR assay. Cytotoxic CTL activity induced by AdVHBsAg-DCs in vitro was detected by MTT assay. Results: Western blotting analysis showed that HBV surface antigen gene was expressed in transfected DCs, suggesting that the transfection was effective. AdVHBsAg-DCs up-regulated the expression of CD1a, CD11c, CD83, CD86 and HLA-DR, but lowered the phagocytosis compared with DC group (P<0.05). Autologous T cells proliferation induced by AdVHBsAg-DCs was obviously higher than DC that in control group and LacZ-DC group (P<0.05). The in vitro cytotoxicity of CTL induced by AdVHBsAg-DCs against HepG2.2.15 cells was specific. Conclusion: Hepatocarcinoma associated antigen HBsAg can be used as a key point in gene therapy of HBV related hepatoma, which provides an experimental base for immunotherapy of HBV related hepatocarcinoma mediated by DCs.