目的： 研究硅和玻璃片基上的三维微结构对乳腺癌MCF-7细胞生长的影响。-方法-： 采用SU-8光刻胶光刻微加工技术在硅和玻璃片基表面制造沟槽、五角星、齿轮状3种三维微结构，与乳腺癌MCF-7细胞共培养，用倒置显微镜与扫描电镜观察三维微结构对肿瘤细胞的形态、分布、生长的影响，采用MTT法分析三维微结构对肿瘤细胞增殖的影响, 用流式细胞仪分析凋亡细胞。结果： 成功制作微米尺度带有不同深宽比的沟槽、五角星、齿轮。显微镜观察显示，MCF-7细胞喜欢在微结构的深槽中生长，特别是边角部位，而微结构的顶部及周边部位细胞非常少；悬浮细胞呈圆形且较多，作为对照的硅片基表面细胞分布稀疏，轻轻晃动后，细胞上浮；而玻璃片基表面对照细胞分布浓密，呈梭形，悬浮细胞很少。MTT分析显示，随着培养时间的增加，玻璃片基与硅片基微结构中细胞增殖抑制率增加显著（P<0.05，P<0.01）。流式细胞仪分析显示，随着培养时间增加，玻璃片基与硅片基上的微结构诱导的凋亡细胞率增加显著（P<0.05，P<0.01）。结论： 在微米尺度带有不同深宽比的沟槽、五角星、齿轮微结构能够抑制MCF-7细胞的生长，并诱导细胞凋亡。此现象在肿瘤治疗方面具有潜在的应用价值。

Objective: To investigate the effects of three-dimensional microstructures of silicon and glass substrates on the growth of breast cancer cell line MCF-7. Methods: Three-dimensional microstructures (grooves, pentagons, and gear wheels) of silicon and glass substrates were fabricated by micro-fabrication technology, and were then co-cultured with MCF-7 cells for 1- 4 days. Phase contrast microscope and scanning electron microscopy were used to observe the distribution, size, shape, and growth of MCF-7 cells, MTT was used to analyze the effects of the microstructures on the proliferation of MCF-7 cells, and flow cytometry was used to analyze the amounts of apoptosis cells. Results: These microstructures, including grooves, pentagram and gear wheels, were successfuly fabricated by micro-fabrication technology. MCF-7 cells were prone to grow on the bottom of grooves, pentagram and gear wheels; few cells were observed on the top and sidewalls of microstructures. Round suspension cells gradually increased as the cell culture time increased. Few cells existed on the surface of control silicon substrate, and the adhesive cells were easily detached from the substrate; however, abundant spindle-shaped MCF-7 cells grew evenly on the surface of glass substrate. MTT analysis showed that as the culture time increased, the inhibition of cell proliferation on the microstructures on the glass and silicon substrates decreased gradually (P<0.01，P<0.05); flow cytometry analysis showed that as the culture time increased, apoptosis cell number also increased gradually (P<0.01，P<0.05). Conclusion: Micron level microstructures, such as grooves, pentagram and gear wheels on the substrates of silicon and glass, can inhibit the growth of MCF-7 cells and induce cell apoptosis, which hold much potential for application in tumor therapy.

国家重点基础研究发展规划（973计划）资助项目（No.2005CB724300G）；上海市科委科学基金资助项目（No.054119527）