目的： 探讨细胞周期蛋白G基因（Cyclin G）mRNA在白血病患者中的表达及其临床意义。方法：采用逆转录聚合酶链反应(RT-PCR)对129例白血病患者和10例正常人白细胞的Cyclin G mRNA表达进行分析。结果：(1)急性白血病（AL）组和慢性白血病（CL）组Cyclin G mRNA的表达值明显高于正常对照组（P<0.05）。但AL组和CL组的差异无显著性（P>005）。(2)AL、AML、ALL组中初治/复发亚组的Cyclin G阳性率分别为71.3%、66.7%和85.7%，均明显高于相应的缓解组（P<0.05或P<0.01）和正常对照组（P<0.01）；但各白血病缓解组与正常对照组之间无显著差异（P>0.05）。(3)Cyclin G阳性表达率在白血病初治患者的不同性别、不同年龄、不同幼稚细胞之间无显著性差异。但AL组和AML组中初治患者WBC计数>50×109/L组的Cyclin G的阳性率明显高于WBC计数≤50×109/L组。(4)初治患者中有53例Cyclin G表达阳性，其中强阳性表达患者的缓解率（51.7%）显著低于一般阳性患者的缓解率（79.1%）（P<0.05）。结论： Cyclin G在白血病患者中有异常高表达，与白血病的发生有一定联系；其异常高表达可能是白血病预后不良的因素之一。

Objective: To investigate the expression of Cyclin G mRNA in leukemia patients and its clinical significance. Methods: RT-PCR was used to analyze the expression of Cyclin G mRNA in the mononuclear cells of 129 leukemia patients and 10 healthy controls. Results: (1) The expression of Cyclin G in acute leukemia (AL) and chronic leukemia (CL) patients was significantly higher than that in healthy controls (both P<0.05), but there was no significant difference between AL and CL patients. (2) The Cyclin G positive rates of newly diagnosed/recurrent cases in AL, acute myeloid leukaemia (AML), acute lymphoblastic leukemia (ALL) groups were respectively 71.3%, 66.7%, and 85.7%, all significantly higher than those of their corresponding remission cases（P<0.05 or P<0.01）and that of healthy controls (P<0.01). (3) The Cyclin G positive rates in newly diagnosed cases of different ages, sexes, and percent of immature cells were similar; the positive rates of newly diagnosed cases with WBC > 50×109/L in AL and AML groups were significantly higher than those with WBC ≤ 50×109/L. (4) Fifty-three of the newly diagnosed cases were Cyclin G positive. The remission rate of patients with high Cyclin G expression（51.7%）was significantly lower than that with low Cyclin G expression（79.1%）(P<0.05). Conclusion: The high expression of Cyclin G may be correlated with the pathogenesis of leukemia and may be one of the factors for the poor prognosis of leukemia.

福建省科技专项基金项目（NO.K04053）