研究蛋白激酶B(protien kinase B，PKB)、缺氧诱导因子1α(hypoxia inducible factor 1α，HIF-1α)在胃癌中的表达及其临床意义。方法： RT PCR法检测26例胃癌及癌旁组织中PKB1、PKB2、PKB3及HIF-1α mRNA表达，免疫组化检测64例胃癌及26例癌旁组织中磷酸化蛋白激酶B(phosphorylated PKB, pPKB)蛋白和HIF-1α蛋白的表达。结果： 1）胃癌及相应癌旁正常组织中PKB1、2、3 mRNA的表达阳性率皆为100%，表达水平差异无显著性(P＞0.05)；胃癌组织中HIF-1α mRNA表达水平显著高于癌旁组织 (1.36±0.14 vs 0.54±0.18, P ＜0.05)。（2）胃癌组织中pPKB、HIF-1α蛋白表达阳性率显著高于癌旁组织(χ2分别为26.936、15.950,P＜0.05)。（3）胃癌组织中pPKB、HIF-1α蛋白表达率均与TNM分期、侵润深度、淋巴结转移、远处转移存在相关性( P ＜0.05)。结论： PKB表达异常发生于翻译或翻译后水平；PKB、HIF-1α过表达于胃癌组织，与胃癌细胞的增殖、凋亡和转移等密切相关。

To investigate the expression of protein kinase B(PKB)and hypoxia inducible factor 1α(HIF-1α) in gastric cancer and its clinical significance. Methods: RT-PCR was used to detect the expression of PKB1,PKB2,PKB3, and HIF-1α mRNA in 26 patients with gastric cancer tissue samples and the corresponding para-cancer tissues. The expression of pPKB and HIF-1α in 64 gastric carcinomas and 26 para-cancer tissue samples were evaluated by immunohistochemical methods. Results: (1) PKB1, PKB2, and PKB3 mRNA were positive in both gastric cancer and the corresponding adjacent normal tissues, with no significant difference ( P ＞0.05). The expression of HIF-1α mRNA in cancerous tissues was significantly higher than that in para-cancer tissues (1.36±0.14 vs 0.54±0.18,P＜0.05). (2)The expression levels of pPKB and HIF-1α proteins in cancerous tissues were higher than those in para-cancer tissues (χ2=26936,15.950, P＜0.05). (3) The expression of pPKB and HIF-1α were significantly associated with TNM stage, invasion depth, lymph node metastasis, and distant metastases (P＜0.05）. Conclusion: The abnormal expression of PKB is at the translation or post-translation level. PKB and HIF-1α are overexpressed in gastric cancer tissues, which is correlatedwith cell proliferation, apoptosis, and metastasis of gastric cancer.

山东省卫生厅科技资助项目(2001CAICJB9)