研究人脂联素重组体对人乳腺癌MDAMB231细胞体外侵袭能力的影响。方法：将MDAMB231细胞分设对照组和脂联素重组体加药(2.5、5.0、10、20、30 μg/ml)组，药物作用一定时间后,分别以MTT法、Transwell小室实验，Chamber小室实验，黏附实验检测脂联素重组体对乳腺癌细胞增殖、侵袭、迁移和黏附能力的影响，以明胶酶谱法检测其对肿瘤细胞分泌MMP2和MMP9的影响。结果：脂联素重组体质量浓度高于5.0 μg/ml时，对MDAMB231细胞生长具有明显的抑制作用（P<0.01）；能明显地降低肿瘤细胞体外侵袭能力（P<0.01），侵袭抑制率随脂联素重组体质量浓度的升高而增加，介于22.64％～77.84％之间；脂联素重组体质量浓度高于2.5 μg/ml时，明显地抑制肿瘤细胞的迁移能力（P<0.01）；脂联素重组体对ECM和FN黏附能力影响的程度不同，对FN的敏感性大于ECM；脂联素重组体质量浓度大于2.5 μg/ml时，明显抑制肿瘤细胞MMP2和MMP9的分泌（P<0.05或P<0.01）,后者的分泌量随质量浓度的升高而下降。结论：脂联素重组体在大于25 μg/ml时，可能通过保护基底膜不受破坏而抑制人乳腺癌细胞的侵袭能力。

To investigate the effects of recombinant adiponectin (RA) on the invasive ability of human breast cancer cell line MDAMB231. Methods: MDAMB231 cells were divided into control group and RA(2.5, 5.0, 10, 20, and 30 μg/ml) treatment groups. The effect of RA on proliferation of MDAMB231 cells was measured by MTT assay; the invasive and migration abilities of MDAMB231 cell were assayed in Transwell cell culture chamber; cell adhesion assay was carried out in a microculture well precoated with fibronectin．Gelatinolytic activities of both MMP2 and MMP9 secreted by cancer cells were measured by zymogrophy analysis. Results: The growth and invasive ability of MDAMB231 cells were obviously inhibited when the concentration of RA was higher than 5.0 μg/ml (P<0.01); the inhibition of migration was in a concentrationdependent manner, with the inhibitory rate ranging from 22.64％77.84％. When the concentration of RA was higher than 2.5 μg/ml, the migration of tumor and the secretion of MMP2 and MMP9 were obviously inhibited (P<0.05,P<0.01); the secretion of MMP9 decreased with the increase of RA concentration. RA was more sensitive to FN and than to ECM. Conclusion: These results indicate that adiponectin is effective in suppressing tumor cell invasion when its concentration is >2.5 μg/ml; the mechanism might be the downregulation of MMPs.

国家自然科学基金资助项目(No. 30500135)