摘 要 目的: 探讨卵巢癌基因1ovarian cancer gene 1,OVCA1;也称为DPH2L( diphthamide synthesis protein 2-like)基因体外对卵巢癌细胞系A2780迁移和侵袭能力的抑制作用。方法：采用脂质体法将GFP标记的携带OVCA1的重组质粒pEGFP-OVCA1或GFP空白质粒分别转染A2780细胞后，G418筛选稳定转染细胞，有限稀释法筛选稳定转染细胞的单克隆细胞株，荧光显微镜检查绿色荧光蛋白的表达及RT-PCR方法检测A2780细胞OVCA1基因mRNA的表达。A2780-OVCA1细胞为实验组， A2780-GFP细胞和A2780细胞 为对照组，采用划痕实验、Transwell体外迁移实验和Matrigel体外侵袭实验检测OVCA1对A2780细胞迁移和侵袭能力的影响。结果：重组质粒pEGFP-OVCA1转染后获得了稳定表达GFP标记OVCA1蛋白的A2780细胞株。A2780-OVCA1组划痕后的细胞迁移速度明显小于两对照组（P<0.05）；A2780-OVCA1组穿过Transwell滤膜的迁移细胞数明显少于两对照组（P<0.05）；A2780-OVCA1组穿过Matrigel滤膜的侵袭细胞数明显少于两对照组（P<0.05）。结论：OVCA1在体外具有显著抑制卵巢癌A2780细胞迁移和侵袭的作用。

Abstract Objective:To investigate the inhibitory effects of tumor suppressor gene OVCA1, also named diphthamide synthesis protein 2like(DPH2L), on migration and invasion of ovarian cancer cell A2780 in vitro. Methods: Ovarian cancer cell line A2780 was transfected with GFPtaggedOVCA1 vector or empty vector via Lipofetamine 2000. Cells stably expressing GFPtaggedOVCA1 (A2780OVCA1) or GFP (A2780GFP) were screened by G418. Stably transfected monoclones were obtained by limiting dilution. GFP protein in the cells was examined by fluorescence microscopy and OVCA1 fragment was examined by RTPCR. Wound healing assay, transwell migration assay and matrigel invasion assay were employed to study the effects of OVCA1 on A2780 cell migration and invasion, while taking A2780GFP and A2780 cells as controls. Results:The cell line stably expressing GFPtagged 〖STBX〗OVCA1〖STBZ〗 (A2780OVCA1) was successfully established. The migration speed of cells in A2780OVCA1 group was significantly lower than those of the other 2 groups (P<0.05). Cells passing the pores of transwell filter was less in A2780OVCA1 cells than in the cells of the 2 control groups (P<0.05). Cells passing matrigelcoated filter was significantly decreased in A2780OVCA1 group than in the 2 control groups (P<0.05). Conclusion: OVCA1 can effectively inhibit A2780 cell migration and invasion in vitro.

辽宁省教育厅资助课题（No.2004F053）