摘 要 目的: 研究微小染色体维持蛋白4(minichromosome maintenance proteins 4,MCM4)、细胞分裂周期蛋白(cell division cycle 6,CDC6)在宫颈癌组织中的表达及其与人乳头状瘤病毒16/18型(human papilloma virus 16/18 type,HPV16/18)感染的相关性及其临床意义。方法：收集2006-2007年间山西大同市第五人民医院病理科经病理证实的50例宫颈癌、20例宫颈上皮内瘤变（cervical intraepithelial neoplasia, CIN）Ⅰ、20例CINⅡ-Ⅲ、20例正常宫颈组织石蜡标本，以免疫组织化学法检测这些组织标本中MCM4、CDC6的表达，同时采用PCR技术检测HPV16/18的感染情况。结果：（1）在宫颈癌组织中MCM4、CDC6表达的阳性率显著高于CIN和正常宫颈组织（均P<0.05），且MCM4、CDC6阳性表达率与宫颈癌病理分级和淋巴转移相关（均P<0.05），而与年龄分组、临床分期无关（均P>0.05）。（2）HPV16/18 感染在正常宫颈组织、CIN和宫颈癌组织中的阳性率依次升高（P<0.05），但与宫颈癌患者年龄、临床分期、病理分级、淋巴转移无关（均P>0.05）。（3）宫颈癌组织中MCM4和CDC6的表达呈正相关（r= 0.390;P<0.05）；MCM4和CDC6表达均与HPV16/18感染相关（r=0.634, P<0.05; r=0.386, P<0.05）。结论：宫颈癌及CIN组织中MCM4、CDC6表达的改变与HPV16/18感染密切相关，共同影响CIN的发展及宫颈癌的发生与发展。

Abstract Objective: To investigate the expression of minichromosome maintenance proteins 4(MCM4) and cell division cycle 6 (CDC6) in uterine cervical carcinomas and its relationship with human papilloma virus (HPV)16/18 infection. Methods：The expression of MCM4 and CDC6 was examined in 50 squamous cell carcinoma specimens, 20 cervical intraepithelial neoplasia (CIN)ⅡⅢ specimens, 20 CINⅠ specimens, and 20 normal cervical tissues by immunohistochemical method. The infections of HPV type 16, 18 DNA were determined by PCR. Results：(1) The expression of MCM4 and CDC6 in uterine cervical carcinoma tissues was significantly higher than that in the CIN specimens and normal cervical tissues (Both P<0.05). Expression of MCM4 and CDC6 was correlated with tumor grades and lymph node metastasis (all P<0.05), but not with age and clinical stages (all P>0.05). (2) The positive rates of (HPV)16/18 were significant different between cervical carcinomas, CIN and normal tissues (P<0.05), and was not associated with age, tumor grades, clinical stages or lymphnode metastasis (all P>0.05). (3) MCM4 expression were positively correlated with the expression of CDC6 in uterine cervical carcimonas（r=0.390, P<0.05）. The positive rate of HPV16/18 was positively correlated with the expression of MCM4 and CDC6 （r= 0.634, P<0.05; r=0.386 P<0.05）.Conclusion: The results suggest that changes in MCM4 and CDC6 expression in CIN and cervical carcinoma specimens are associated with HPV16/18 infection, which is related to the progression of CIN and carcinogenesis of cervical carcinomas.