摘 要 目的: 检测血管内皮生长因子（vascular endothelial growth factor，VEGF）基因在子宫内膜癌、癌周组织、正常子宫内膜及外周血循环中的表达情况，分析其在肿瘤生长、转移中的作用。方法: 采用荧光实时定量PCR方法检测51例子宫内膜癌患者的癌组织和癌周组织、40例正常子宫内膜组织及其对应的患者外周血中的VEGF表达情况，分析其与临床病理参数之间的关系。结果: 子宫内膜癌组织中VEGF表达水平显著高于癌周组织及正常子宫内膜组织(P<0.05) ；与临床分期、组织学分级、淋巴结转移、肌层浸润深度密切相关 (均P<0.05)，但与肿瘤病理类型及患者是否绝经无明显相关性(P>0.05)。子宫内膜癌患者外周血中VEGF的表达明显高于正常对照(P>0.05)，且与临床分期、组织学分级、病理类型及淋巴结转移有显著相关(均P<0.05)，但与肌层浸润程度及患者是否绝经无明显相关性。结论：荧光实时定量PCR可以敏感、特异性地检测子宫内膜癌组织及外周血中VEGF的表达，VEGF在子宫内膜癌的发生、侵袭、转移过程中可能起重要作用。

Abstract Objective: To examine the expression of VEGF gene in the endometrial carcinoma tissues, paratumor tissues, normal endometria and peripheral blood, and analyze the role of VEGF in tumor growth and tumor metastasis. Methods: Realtime fluorescence quantitative PCR was used to detect the expression of VEGF gene in 51 endometrial carcinoma samples and the corresponding paratumor tissues, 40 normal endometria samples and their corresponding peripheral blood samples. The relation between the VEGF expression and clinical pathological parameters was analyzed.Results: The expression of VEGF gene was higher in the endometrial carcinoma tissues than in the corresponding paratumour tissues and normal endometrial tissues (P<0. 05) . VEGF expression in endometrial carcinoma was significantly correlated with the clinical stage, histological grade, lymph node metastasis and depth of myometrial invasion (all P<0.05), but not with the presence of menopause or the pathological types of the tumor (P>0.05). The expression of VEGF in peripheral blood was higher in patients with endometrial carcinoma than that in the normal controls; and the expression was significantly correlated with the clinical stage, histological grades, pathological types and the presence of lymph node metastasis (P<0.05), but not with the depth of myometrial invasion and the presence of menopause( P>0.05). Conclusion: Realtime fluorescent quantitative PCR can sensitively, specifically detect the expression of VEGF in the endometrial carcinoma tissues and peripheral blood samples. VEGF might play an important role in the development, invasion, and metastasis of endometrial carcinoma.

青岛市科技局发展基金资助(No.051NS77)